国際ジャーナル

  • [2021]
    "Toward the development of a label-free multiple immunosensor based on thin film transistor microelectrode arrays",
    Dongchen Zhu, Grant A. Cathcart, Satoshi Ihida, Hiroshi Toshiyoshi, Agnès Tixier-Mita,
    Yasuyuki Sakai, and Kikuo Komori
    Journal of Micromechanics and Microengineering, in press.

  • "Bioinspired perfluorocarbon-based oxygen carriers with concave shape
    and deformable shell"
    Xiaoting Fu, Seiichi Ohta, Takahiro Kawakatsu, Masamichi Kamihira, Yasuyuki
    Sakai, Taichi Ito
    Advanced Materials Technologies, in press.

  • A Kinetic‐Pump Integrated Microfluidic Plate (KIM‐Plate) with High Usability for Cell Culture‐based Multi‐Organ Microphysiological Systems
    Kenta Shinha, Wataru Nihei, Hiroko Nakamura, Tomomi Goto, Takumi Kawanishi, Naoki Ishida, Nao Yamazaki, Yuki Imakura, Shinji Mima, Kosuke Inamura, Hiroshi Arakawa, Masaki Nishikawa, Yukio Kato, Yasuyuki Sakai, Hiroshi Kimura
    Micromecanics, in press.

  • Differentiation of human induced pluripotent stem cell-derived endocrine progenitors to islet-like cells using dialysis suspension culture system
    Hyunjin CHOI, Marie SHINOHARA, Masato IBUKI, Masaki NISHIKAWA, Yasuyuki SAKAI
    Cellis, in press

  • Characterization of the proteome and metabolome of human liver sinusoidal endothelial-like cells derived from induced pluripotent stem cells
    Mathieu Danoy, Rachid Jellali, Yannick Tauran, Johanna Bruce, Marjorie Leduc, Françoise Gilard, Bertrand Gaki`ere, Benedikt Scheidecker, Taketomo Kido, Atsushi Miyajima, Fabrice Soncin, Yasuyuki Sakai, Eric Leclerc
    Differentiation 120 (2021) 28–35.

  • Investigation of the hepatic development in the coculture of hiPSCs-derived LSECs and HLCs in a fluidic microenvironment
    Mathieu Danoy, Yannick Tauran, Stephane Poulain, Rachid Jellali, Johanna Bruce, Marjorie Leduc, Morgane Le Gall, Yuta Koui, Hiroshi Arakawa, Francoise Gilard, Bertrand Gakiere, Yukio Kato, Charles Plessy, Taketomo Kido, Atsushi Miyajima, Yasuyuki Sakai, Eric
    Leclerc
    APL Bioengineering 5, 026104 (2021); https://doi.org/10.1063/5.0041227

  • Prospect of in vitro bile fluids collection in improving cell based assay of liver function
    Astia Rizki Safitri, Fumiya Tokito, Masaki Nishikawa, Minoru Tanaka, Kazuya Maeda, Hiroyuki Kusuhara and Yasuyuki Sakai
    Front. Toxicol.,03 june 2021;doi: 10.3389/ftox.2021.657432.

  • Fabrication of a porous three-dimensional scaffold with inter-connected
    flow channels: co-cultured liver cells and in-vitro haemocompatibility
    assessment
    Muxin Li, Rubina Rahaman Khadim *, Mitsuru Nagayama, Marie, Shinohara,
    Kousuke Inamura, Mathieu Danoy, Masaki Nishikawa, Katsu Furukawa, Yasuyuki
    Sakai, Toshiki Niino
    Appl. Sci. 2021, 11(6), 2473;https://doi.org/10.3390/app11062473
    *Equal contribution

  • Analysis of the behavior of 2D monolayers and 3D spheroid human pancreatic beta cells derived from induced pluripotent stem cells in a microfluidic environment
    Amal Essaouiba, Rachid Jellali, Marie Shinohara, Benedikt Scheidecker,
    Cecile Legallais, Yasuyuki Sakai, Eric Leclerc
    Journal of Biotechnology,2021 330:25-56.

  • Application of a Thin-Film Transistor Array for Cellular-Resolution
    Electrophysiology and Electrochemistry
    Anne-Claire Eiler, Pierre-Marie Faure, Junichi Sugita, Satoshi Ihida, Dongchen Zhu, Yasuyuki Sakai,
    Katsuhito Fujiu, Kikuo Komori, Hiroshi Toshiyoshi, and Agnes Tixier-Mita,
    IEEE Transactions on Electron Devices,68(4), 2041-2048 (2021); doi: 10.1109/TED.2021.3050432.

  • Enhancement of human hepatocyte functions by coculture with hiPS-derived intestinal
    cells in a pneumatic-pressure-driven two-organ microphysiological system
    Marie Shinohara, Hiroshi Arakawa, Yuuichi Oda, Nobuaki Shiraki, Shinji
    Sugiura, Takumi Nishiuchi, Taku Satoh, Keita Iino, Sylvia Leo, Yusuke
    Kato, Karin Araya, Takumi Kawanishi, Took Nakatsuji, Manami Mitsuta,
    Kosuke Inamura, Tomomi Goto, Kenta Shinha, Wataru Nihei, Kikuo Komori,
    Masaki Nishikawa, Shoen Kume, Yukio Kato, Toshiyuki Kanamori, Yasuyuki
    Sakai, Hiroshi Kimura
    Sci. Rept.,11, 5437 (2021); https://doi.org/10.1038/s41598-021-84861-y.

  • Multi-omics analysis of hiPSCs-derived HLCs matured on-chip revealed patterns typical of liver regeneration
    Danoy, Mathieu; Tauran, Yannick; Poulain; Jellali, Rachid; Bruce,
    Johanna; Leduc, Marjorie; Le Gall; Gilard, Francoise; Kido, Taketomo;
    Arakawa, Hiroshi; Araya, Karin; Mori, Daiki; Kusuhara, Hiroyuki; Kato,
    Yukio; Plessy, Charles; Miyajima, Atsushi; Sakai, Yasuyuki; Leclerc,
    Eric
    Biotecnol. Booeng.,2021; 1– 17; https://doi.org/10.1002/bit.27667.

  • High density culture of pancreatic islet-like 3D tissue organized in oxygen-permeable porous scaffolds with external oxygen supply
    Fumiya Tokito, Marie Shinohara, Masashi Maruyama, Kosuke Inamura, Masaki
    Nishikawa, Yasuyuki Sakai
    J. Biosci. Bioeng.,131(5), 543-548 (2021); https://doi.org/10.1016/j.jbiosc.2020.12.009

  • Sessile liquid marbles with embedded hydrogels as 1 bioreactors for three-dimensional cell
    culture
    Raja Vadivelu, Navid Kashaninejad, Mohammad Reza Nikmaneshi, Rubina Rahaman Khadim,
    Seyedeh Sarah Salehi, Naveen Chintala Ramulu, Yasuyuki Sakai, Masaki
    Nishikawa, Bahar Firoozabadi, Nam-Trung Nguyen
    Advanced Biology, 5: 2170022; https://doi.org/10.1002/adbi.202170022

  • 3D perfusion culture of mouse insulinoma in macro-porous scaffolds enhanced insulin production response
    Changsorn, Karn; Pang, Yuan; Matsumoto, Hiroaki; Wuthrich, Pierre; Sun, Wei;
    Sakai, Yasuyuki,
    The International Journal of Artificial Organs, in press.

  • Bioelectrochemical detection of histamine release from basophilic leukemia cell line based on histamine dehydrogenase-modified cup-stacked carbon nanofibers
    Kikuo Komori, Yuto Komatsu, Masaharu Nakane, Yasuyuki Sakai
    Bioelectrochemistry, 138 (2021) 107719.
  • [2020]
    Microfluidic System with Extended‐Gate‐Type Organic Transistor for Real‐Time Glucose Monitoring
    Pierre Didier, Nicolas Lobato‐Dauzier, Nicolas Clément, Anthony J. Genot, Yui Sasaki, Éric Leclerc, Tsukuru Minamiki, Yasuyuki Sakai, Teruo Fujii, Tsuyoshi Minami
    ChemElectroChem, 7, 1332 –1336 (2020); https://doi.org/10.1002/celc.201902013

  • Development of a pancreas-liver organ-on-chip coculture model for organ-to-organ interaction studies
    Amal Essaouiba, Teru Okitsu, Rie Kinoshita, Rachid Jellali, Marie Shinohara, Mathieu Danoy, CécileLegallais, Yasuyuki Sakai, Eric Leclerc
    Biochemical Engineering Journal, 164, 107783 (2020); https://doi.org/10.1016/j.bej.2020.107783

  • Microwell-based pancreas-on-chip model enhances genes expression and functionality of rat islets of Langerhans
    Amal Essaouiba, Teru Okitsu, Rachid Jellali, Marie Shinohara, Mathieu Danoy, Yannick Taurane, Cécile Legallais, Yasuyuki Sakai, Eric Leclerc
    Molecular and Cellular Endocrinology, 514, 110892 (2020); https://doi.org/10.1016/j.mce.2020.110892

  • Modular assembly–based approach of loosely packing co-cultured hepatic tissue elements with endothelialization for liver tissue engineering
    Jianyu He, Yuan Pang, Huayu Yang, Kevin Montagne, Marie Shinohara, Yilei Mao, Wei Sun, Yasuyuki Sakai
    Annals of Translational Medicine, in press.

  • Protection of human induced pluripotent stem cells against shear stress in suspension culture by Bingham plastic fluid
    Ikki Horiguchi, Fuad Gandhi Torizal, Hotaka Nagate, Haruka Inose, Kousuke Inamura, Osamu Hirata, Hisato Hayashi, Masato Horikawa, Yasuyuki Sakai
    Biotechnology Progress, in press.

  • Kinetic analysis of sequential metabolism of triazolam and its extrapolation to humans using an entero-hepatic two-organ microphysiological system
    Hiroshi Arakawa, Shinji Sugiura, Takumi Kawanishi, Kazumi Shin, Hiroko Toyoda, Taku Satoh, Yasuyuki Sakai, Toshiyuki Kanamori, Yukio Kato
    Lab Chip, 2020,20, 537-547, https://doi.org/10.1039/C9LC00884E

  • Analysis of hiPSCs differentiation toward hepatocyte-like cells upon extended exposition to oncostatin
    Mathieu Danoy, Yannick Tauran, Stephane Poulain, Hiroshi Arakawa, Daiki Mori, Karin Araya, Sachi Kato, Taketomo Kido, Hiroyuki Kusuhara, Yukio Kato, Atsushi Miyajima, Charles Plessy, Yasuyuki Sakai, Eric Leclerc
    Differentiation, 114, 36-48 (2020); https://doi.org/10.1016/j.diff.2020.05.006

  • Design, fabrication, and evaluation of polyglycolic acid modules with canals as tissue elements in cellular assembly technology
    Jingyuan Ji, Yuan Pang *, Stephanie Sutoko, Yohei Horimoto, Wei Sun, Toshiki Niino, Yasuyuki Sakai
    Applied Sciences, 10(11), 3748 (2020); https://doi.org/10.3390/app10113748

  • Induction of in vitro Metabolic Zonation in Primary Hepatocytes Requires Both Near-Physiological Oxygen Concentration and Flux
    Benedikt Scheidecker, Marie Shinohara, Masahiro Sugimoto, Mathieu Danoy, Masaki Nishikawa, and Yasuyuki Sakai
    Frontiers in Bioengineering and Biotechnology, section Tissue Engineering and Regenerative Medicine, 8, 524 (2020); https://doi.org/10.3389/fbioe.2020.00524

  • A tubular bile duct structure mimicking bile duct morphogenesis for prospective in vitro liver metabolite recovery
    Astia Rizki-Safitri, Marie Shinohara, Minoru Tanaka, and Yasuyuki Sakai
    Journal of Biological Engineering, 2020 Mar 19;14:11

  • Characterization of liver zonation-like transcriptomic patterns in HLCs derived from hiPSCs in a microfluidic biochip environment
    Mathieu Danoy, Stephane Poulain, Myriam Lereau-Bernier, Sachi Kato, Benedikt Scheidecker, Taketomo Kido, Atsushi Miyajima, Yasuyuki Sakai, Charles Plessy, Eric Leclerc
    Biotechnology Progress, e3013 (2020)

  • Integration of metabolomic and transcriptomic profiles of hiPSCs-derived hepatocytes in a microfluidic environment
    Danoy, M., Poulain, S., Jelalli, R., Gilard, F., Kato, S., Plessy, C., Kido, T., Miyajima, A., Sakai, Y., & Leclerc, E.
    Biochemical Engineering Journal, 155, 107490,(2020)

  • Transcriptome profiling of hiPSCs-derived LSECs with nanoCAGE
    Danoy, M., Poulain, S., Koui, Y., Tauran, Y., Kido, T., Miyajima, A., Sakai, Y., Plessy, C., & Leclerc, E
    Molecular Omics, 2020, Advance Article, (2020)

  • Organization of liver organoids using Raschig ring-like micro-scaffolds and triple co-culture: Toward modular assembly-based scalable liver tissue engineering
    Pang, Y., Sutoko, S., Wang, Z., Horimoto, Y., Montagne, K., Horiguchi, I., Shinohara, M., Danoy, M., Niino, T., Sakai, Y
    Medical Engineering & Physics, 76, 69-78 (2020)

  • Integration of metabolomic and transcriptomic profiling to compare two protocols of differentiation of human induced pluripotent stem cells into hepatocytes
    Jellali, R., Poulain, S., Bernier, M. L., Gilard, F., Tauran, Y., Kato, S., Danoy, M., Segard, B., D., Kido, T., Miyajima, A., Plessy, C., Sakai, Y., Leclerc, E.
    Process Biochemistry, 88, 138-147, (2020)

  • Metabolomic profiling during the differentiation of human induced pluripotent stem cells into hepatocyte-like cells,
    Jellali, R., Bernier, M. L., Tauran, Y., Gilard, F., Danoy, M., Kido, T., Miyajima, A., Sakai, Y., Leclerc, E..
    Differentiation. 112, 17-26 (2020)
  • [2019]
  • Cytoplasmic fusion between an enlarged embryonic stem cell and a somatic cell using a microtunnel device
    Seong Min Kim; Ken-Ichi Wada; Masashi Ueki; Kazuo Hosokawa; Yasuyuki Sakai; Mizuo Maeda; Yoshihiro Ito
    Biochemical and Biophysical Research Communications (BBRC), 520, 257-262 (2019)
  • Improved isolation of mesenchymal stem cells based on interactions between N-acetylglucosamine-bearing polymers and cell-surface vimentin
    Hirohiko Ise, Kumiko Matsunaga, Marie Shinohara and Yasuyuki Sakai
    Stem Cells International, vol. 2019, 13, (2019)

  • Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device.
    Matsumoto S, Safitri AR, Danoy M, Maekawa T, Kinoshita H, Shinohara M, Sakai Y, Fujii T, Leclerc E.
    Biotechnol Prog. 2019 May 26:e2854.

  • Micropatterned nanolayers immobilized with nerve growth factor for neurite formation of PC12 cells,
    Seong Min Kim, Masashi Ueki, Xueli Ren, Jun Akimoto, Yasuyuki Sakai, and Yoshihiro Ito
    International Journal of Nanomedicine, 14, 2019, 7683-7694 (2019)
  • Metabolome Analysis Reveals Dermal Histamine Accumulation in Murine Dermatitis Provoked by Genetic Deletion of P-Glycoprotein and Breast Cancer Resistance Protein.
    Hashimoto N, Nakamichi N, Nanmo H, Kimura KI, Masuo Y, Sakai Y, Schinkel AH, Sato S6, Soga T, Kato Y.
    Pharm Res. 2019 Sep 11;36(11):158. (2019)

  • Biodegradable and hollowed micro-scaffolds for improved modular assembly-based tissue engineering: design, 3D fabrication, and feasibility in randomly packed perfusion culture,
    Stephanie Sutoko, Yuan Pang, Yohei Horimoto, Kevin Montagne, Kikuo Komori, Kiyoshi Takano, Ryo Shirakashi, Masahiro Anzai, Toshiki Niino, Yasuyuki Sakai,
    Biochem. Eng. J., 149, 107239 (2019)

  • Improvement of oxygen supply to the multicellular spheroids using a gas-permeable plate and embedded hydrogel beads,
    Hirotaka Mihara , Mai Kugawa , Kanae Sayo , Fumiya Tao , Marie Shinohara , Masaki Nishikawa , Yasuyuki Sakai , Takeshi Akama , Nobuhiko Kojima,
    Cells, 2019, 8, 525 (2019)

  • Switching of cell proliferation/differentiation in thiol-maleimide clickable microcapsules triggered by in situ conjugation of biomimetic peptides,
    Oki, Yuichiro; Kirita, Katsuhisa; Ohta, Seiichi; Ohba, Shinsuke; Horiguchi, Ikki; Sakai, Yasuyuki; Ito, Taichi,
    Biomacromolecules, 2019, 20, 6, 2350-2359 (2019)

  • Size-Dependent Hepatic Differentiation of Human Induced Pluripotent Stem Cells Spheroid in Suspension Culture,
    Fuad Gandhi-Torizal, Keiichi Kimura, Ikki Horiguchi, Yasuyuki Sakai,
    Regen. Ther, 12, 66-73 (2019)

  • A novel tool for suspension culture of human induced pluripotent stem cells: Lysophospholipids as a cell aggregation regulator
    M. Ibuki, I. Horiguchi, Y. Sakai
    Regen. Ther, 12, 74-82 (2019)
  • Endodermal differentiation of human induced pluripotent stem cells using simple dialysis culture system in suspension culture
    M. Shinohara, H. Choi, M. Ibuki, S. G.Yabe, H. Okochi, A. Miyajimam Y. Sakai
    Regen. Ther, 12, 14-19 (2019)

  • Size-controlled preparation of microsized perfluorocarbon emulsions as oxygen carriers via the Shirasu porous glass membrane emulsification technique
    X. T. Fu, S. Ohta, M. Kamihira, Y. Sakai and T. Ito
    Langmuir, 35 (11), pp 4094-4100, (2019)

  • Analysis of the transcription factors and their regulatory roles during a step-by-step differentiation of induced pluripotent stem cells into hepatocyte-like cells
    Tauran, Y., Poulain, S., Lereau-Bernier, M., Danoy, M., Shinohara, M., Segard, B. D., Kato, S., Kido, T., Miyajima, A., Sakai, Y., Plessy, C., Leclerc, E.
    Molecular omics, 15(6), 383-398 (2019)

  • Optimized protocol for the hepatic differentiation of iPSCs in a fluidic microenvironment
    Mathieu Danoy, Myriam Lereau Bernier, Keiichi Kimura, Stephane Poulain,
    Sachi Kato, Daiki Mori, Taketomo Kido, Charles Plessy, Hiroyuki Kusuhara,
    Atsushi Miyajima, Yasuyuki Sakai, Eric Leclerc
    Biotechnology and Bioengineering, 116(7), 1762-1776 (2019)

  • Profiling of derived-hepatocyte progenitors from induced pluripotent stem cells using nanoCAGE promoter analysis
    Myriam Lereau Bernier, Stéphane Poulain, Yannick Tauran, Mathieu Danoy, Marie Shinohara, Keiichi Kimura, Bertrand David Segard, Sachi Kato, Taketomo Kido, Atsushi Miyajima, Yasuyuki Sakai, Charles Plessy, Éric Leclerc
    Biochemical engineering journal, 142, 7-17 (2019)

  • Physiological Microenvironmental Conditions in Different Scalable Culture Systems for Pluripotent Stem Cell Expansion and Differentiation
    Fuad G. Torizal, Ikki Horiguchi, Yasuyuki Sakai
    The Open Biomedical Engineering Journal., 13, 41-54 (2019)

  • Enhanced hepatic differentiation of human iPS cells using gas permeable membrane
    Keiichi Kimura, Ikki Horiguchi, Taketomo Kido, Atsushi Miyajima, Yasuyuki Sakai 
    Tissue Eng. Part A, 25, 5-6 (2019)

  • An orbital shaking culture of mammalian cells in O-shaped vessels for the production of uniform aggregates
    Ikki Horiguchi, Ikumi Suzuki, Takashi Morimura, Yasuyuki Sakai
    J. Vis. Exp., (143), e57922, doi:10.3791/57922 (2019).
  • [2018]
  • Integration of an oxygen sensor into polydimethylsiloxane hepatic culture device for two-dimensional gradiate characterization,
    T. Matsumoto, E. Leclerc, T. Mekawa, H. Kinoshita, M. Shinohara, K. Komori, Y. Sakai, T. Fujii,
    Sens. Actuat. B Chem., 273, 1062-1069 (2018).

  • Development of human-derived hemoglobin/albumin microspheres as oxygen carriers using Shirasu porous glass membrane emulsification,
    S. Ohta, K. Hashimoto, X. T. Fu, M. Kamihira, Y. Sakai, and T. Ito,
    Journal of Bioscience and Bioengineering, 126 (4), pp 533-539, (2018).

  • Efficient functional cyst formation of biliary epithelial cells using microwells for potential bile duct organisation in vitro
    A. Rizki-Safitri, M. Shinohara, Y. Miura, M Danoy, M Tanaka, A Miyajima, Y. Sakai
    Scientific Reports, 8(1), 11086 (2018).

  • Effects of glucose, lactate and basic FGF as limiting factors on the expansion of human induced pluripotent stem cells
    Ikki Horiguchi, Yusuke Urabe, Keiichi Kimura, Yasuyuki Sakai
    J. Biosci. Bioeng., 125(1), 111-115 (2018).
  • [2017]
    Enhanced self-organization of size-controlled hepatocyte aggregates on oxygen permeable honeycomb microwell sheets
    Marie Shinohara, Kikuo Komori, Teruo Fujii, Yasuyuki Sakai
    Biomedical Physics & Engineering Express, 3(4), 045017 (2017).
  • Alteration of pancreatic carcinoma and promyeloblastic cell adhision in liver microvasculature by coculture of hepatocytes, hepatic stellate cells and endothelial cells in a physiologically-relevant model
    Mathieu Danoy, Marie Shinohara, Astia Rizki-Safitri, Dominique Collard, Vincent Senez, Yasuyuki Sakai
    Integrative Biology, 9(4), 350-361 (2017).
  • Comparison of the transcriptomic profile of hepatic human induced pluripotent stem like cells cultured in Petri and in a 3D microscale dynamic environment
    Eric Leclerc, Keiichi Kimura, Marie Shinohara, Mathieu Danoy, Morgane Le Galld, Taketomo Kido, Atsushi Miyajima, Teruo Fujii, Yasuyuki Sakai
    Genomics, 109(1), 16-26 (2017).
  • [ 2016 ]
    Novel integrative methodology for engineering large liver tissue equivalents based on three-dimensional scaffold fabrication and cellular aggregate assembly
    Y. Pang, Y. Horimoto, S. Sutoko, K. Montagne, M. Shinohara, M. Danoy, K. Komori, M. Anzai, T. Niino, Y. Sakai
    Biofabrication, 8(3), 035016 (2016)
  • Direct Electron Transfer Kinetics of Peroxidase at Edge Plane Sites of Cup-Stacked Carbon Nanofibers and their Comparison with Single-Walled Carbon Nanotubes
    K. Komori, T. Tatsuma, Y. Sakai
    Langmuir, 32, 9163-9170 (2016).
  • Serum replacement with albumin-associated lipids prevents excess aggregation and enhances growth of induced pluripotent stem cells in suspension culture
    Ikki Horiguchi and Yasuyuki Sakai
    Biotechnology progress, 32(4), 1009-16 (2016).
  • Highly Efficient and Gentle Trapping of Single Cells in Large Microfluidic Arrays for Time-lapse Experiments
    Filiz Yesilkoy, Ryohei Ueno, Benoit Xavier Emmanuel Desbiolles, Marco Grisi, Yasuyuki Sakai, Beomjoon Kim, Juergen Brugger
    Biomicrofluidics, 10(1), 014120 (2016).
  • Oxygenated Cup-Stacked Carbon Nanofibers/TiO2 Composite Films with Enhanced Photocatalytic Currents
    K. Komori, K. Yamura, A. Kogo, Y. Takahashi, T. Tatsuma, A. Sakoda, Y. Sakai
    Bull. Chem. Soc. Japan., 89, 603-607 (2016).
    Selected paper of issue
  • Production of cisplatin-incorporating hyaluronan nanogels via chelating ligand-metal coordination.
    S. Ohta, S. Hiramoto, Y. Amano, M. Sato, Y. Suzuki, M. Shinohara, S. Emoto, H. Yamaguchi, H. Ishigami, Y. Sakai, J. Kitayama, T. Ito, Bioconjug. Chem., 27(3), 504-8 (2016).
  • Three-dimensional graphene: biocompatible and biodegradable scaffold with enhanced oxygenation for HepG2 liver cells culture
    Manuela Loeblein, Guillaume Perry, Siu Hon Tsang, Wenjin Xiao, Dominique Collard, Philippe Coquet, Yasuyuki Sakai, Edwin Hang Tong Teo*
    Advanced Healthcare Materials, 5(10), 1177-91 (2016).

  • [ 2015 ]
    New physiologically-relevant liver tissue model based on hierarchically cocultured primary rat hepatocytes with liver endothelial cells.
    Xiao W., Perry G., Komori K., Sakai Y., Inteqr. Biol., 11, 1412-22, (2015)
  • Establishment of a new liver tissue model by hierarchically coculturing primary rat hepatocytes with liver sinusoidal endothelial cells on a gas-permeable membrane
    Wenjin XIAO, Guillaume PERRY, Kikuo KOMORI, Yasuyuki SAKAI
    Integrative Biology, 7(11), 1412-22 (2015).
  • Alginate encapsulation of pluripotent stem cells using a co-axial nozzle
    I. Horiguchi and Y. Sakai
    J. Visualized. Exp., 101, e52835 (2015).
  • Size-dependent interaction of cells and hemoglobin-albumin based oxygen carriers prepared using the SPG membrane emulsification technique.
    Lai YT, Ohta S, Akamatsu K, Nakao SI, Sakai Y, Ito T. Biotechnol. Prog., 31(6), 1676-84 (2015).
  • Preparation of uniform-sized hemoglobin-albumin microspheres as oxygen carriers by Shirasu porous glass membrane emulsification technique
    Lai YT, Sato M, Ohta S, Akamatsu K, Nakao SI, Sakai Y, Ito T.
    Colloids. Surf. B Biointerfaces., 127, 1-7 (2015).
  • [ 2014 ]
    Wenjin XIAO, Hitoshi MATSUI, Marie SHINOHARA, Kikuo KOMORI, Tomoharu OSADA, Yasuyuki SAKAI
    "The importance of physiological oxygen concentrations in the sandwich cultures of rat hepatocytes on gas-permeable membranes"
    Biotechnology Progress, 2014 Nov-Dec;30(6):1401-10.
  • Ikki Horiguchi, Mohammad M. Chowdhury, Yoji Tabata, Yasuyuki Sakai
    "Proliferation, morphology and pluripotency of mouse induced pluripotent stem cells (iPSCs) in three different types of alginate beads for mass production"
    Biotechnol. Prog.,2014 Jul-Aug;30(4):896-904.
  • Mohammad Mahfuz Chowdhury, Mathieu Danoy, Farhana Rahman, Marie Shinohara, Shohei Kaneda, K. Shiba, Naoya Fujita, Teruo Fujii and Yasuyuki Sakai
    "Adhesion of pancreatic cancer cells in a liver-microvasculature mimicking co-culture correlates with their propensity to form liver-specific metastasis in vivo"
    BioMed Research International, Special issue on "Hepatocellular Carcinoma: Carcinogenesis, Establishment, Progression, and Therapies", in press.
  • Kojima, N., Takeuchi, S. and Sakai, Y.
    "Fabrication of microchannel networks in multicellular spheroids"
    Sensors and Actuators B 198 (2014) 249–254.
  • S. Ko, T. Tatsuma, A. Sakoda, Y. Sakai, and K. Komori, "Electrochemical
    Properties of Oxygenated Cup-Stacked Carbon Nanofiber-Modified Electrodes",
    Phys. Chem. Chem. Phys.,2014 Jun 28;16(24):12209-13
  • N. Kojima, S. Takeuchi Y. Sakai
    "Engineering of Pseudoislets: Effect on Insulin Secretion Activity by
    Cell Number, Cell Population, and Microchannel Networks"
    Transplant. Proc.,2014 May;46(4):1161-5. doi: 10.1016
  • H. Nakayama, H. Kimura, T. Fujii, Y. Sakai
    "Image-based evaluations of distribution and cytotoxicity of Irinotecan
    (CPT-11) in a multi-compartment micro-cell coculture device"
    J. Biosci. Bioeng.,2014 Jun;117(6):756-62.
  • Y. Tabata, I. Horiguchi, M. P. Lutolf, Y. Sakai
    "Development of bioactive hydrogel capsules for the 3D expansion of pluripotent stem cells in bioreactors"
    Biomat. Sci.,2,176-183 (2014).
  • M. Shinohara, H. Kimura, K. Montagne, K. Komori, T. Fujii, Y. Sakai
    "Combination of microwell structures and direct oxygenation enables efficient
    and size-regulated aggregate formation of an insulin-secreting pancreatic beta-cell line"
    Biotechnol. Prog.,30, 178-187, (2014).

  • [ 2013 ]
  • T. Katsuda, N. Kojima, T. Ochiya and Y. Sakai
    "Biliary epithelial cells play an essential role in the reconstruction of hepatic tissue with a functional bile ductular network"
    Tissue Eng. A, 19(21-22), 2402-2411 (2013).
  • N. Kojima, Y. Sakai
    "Control of liver tissue reconstitution in mesenteric leaves: the effect of preculture on mouse hepatic progenitor cells prior to transplantation"
    J. Robotics Mechatronics, 25(4), 698-704 (2013).
  • K. Iwasawa, G. Tanaka, T. Aoyama, M. M. Chowdhury, K.Komori, T. Tanaka-Kagawa, H. Jinno, Y. Sakai
    "Prediction of phthalate permeation through pulmonary alveoli using a cultured A549 cell-based in vitro alveolus model and a numerical simulation", AATEXm Vol. 18 (2013) No. 1 p. 19-31
  • YT. Lai, S. Ohta, K. Akamatsu, S. Nakao Y. Sakai, T. Ito
    "Preparation of uniform-sized poly[methacryloxypropyl tris(trimethylsiloxy)silane] microspheres via Shirasu porous glass membrane emulsification technique"
    J. Chem. Eng. Jpn.,Vol. 46 (2013) No. 11 p. 777-784
  • K. Komori, M. Udagawa, M. Shinohara, K. Montagne, T. Tsuru, and Y. Sakai
    “Formation and Harvesting of Thick Pancreatic β-Cell Sheets on a Highly
    O2-Permeable Plate Modified with Poly(N-isopropylacrylamide)”, Biomaterials
    Science, 1, 510-518 (2013).
  • T. Katsuda, R.Tsuchiya, N. Kosaka, Y.Yoshioka, K. Takagaki, K.Oki, F.Takeshita, Y. Sakai, M.Kuroda and T Ochiya
    "Human adipose tissue-derived mesenchymal stem cells secrete functional neprilysin-bound exosomes"
    Scientific Reports (2013), DOI: 10.1038/srep01197
  • T. Katsuda, T.Teratani, M. M. Chowdhury, T. Ochiya and Y.Sakai
    "Hypoxia efficiently induces differentiation of mouse embryonic stem cells into endodermal and hepatic progenitor cells"
    Biochemical Engineering Journal 74 95-101 (2013).
  • M. M. Chowdhury, T. Fujii and Y. Sakai
    "Importance of a diffusion-dominant small volume to activate cell-secreted soluble factor signaling in embryonic stem cell culture in microbioreactors: A mathematical model based study"
    Journal of Bioscience and Bioengineering (2013),DOI: 10.1016/j.jbiosc.2013.01.008.
  • [ 2012 ]
  • Y. Pang, K. Montagne, M. Shinohara, K. Komori, and Y. Sakai
    "Liver tissue engineering based on aggregate assembly: efficient formation of endothelialized rat hepatocyte aggregates and their immobilization with biodegradable fibers"
    Biofabrication, 4 (2012) 045004.
  • J. Kawada, H. Kimura, H. Akutsu, Y. Sakaiab and T. Fujii
    "Spatiotemporally controlled delivery of soluble factors for stem cell differentiation"
    Lab Chip, 12 (21), 4508 - 4515 (2012).
  • S. Ko, Y. Takahashi, A. Sakoda, Y. Sakai, and K. Komori*
    "Direct Synthesis of Cup-Stacked Carbon Nanofiber Microspheres by the Catalytic Pyrolysis of Poly(ethylene glycol)"
    Langmuir, 28 (23), 8760–8766 (2012).
  • M. M. Chowdhury, H.Kimura, T. Fujii, and Y. Sakai,
    "Induction of alternative fate other than default neuronal fate of embryonic stem cells in a membrane-based two-chambered microbioreactor
    by cell-secreted BMP4"
    Biomicrofluidics, 6, 014117 (2012).
  • K. Komori, S. Fujii, K. Montagne, H. Nakamura, H. Kimura, K. Otake, T.Fujii, and Y. Sakai,
    “Development of the Well of the Well System-Based Embryo Culture Plate with an Oxygen Sensing Photoluminescent Probe”
    Sens. Actuators B, 162, 278-283 (2012).
  • S. Ko, Y. Takahashi, H. Fujita, T. Tatsuma, A. Sakoda, and K. Komori
    "Peroxidase-Modified Cup-Stacked Carbon Nanofiber Networks for Electrochemical Biosensing with Adjustable Dynamic Range"
    RSC Advances, 2, 1444-1449 (2012).
  • F. Evenou, M. Hamon, T. Fujii, S. Takeuchi, and Y. Sakai
    "Gas-permeable Membranes and Co-culture with Fibroblasts Enable High-density Hepatocyte Culture as Multilayered Liver Tissues"
    Biotechnol. Prog. 27, 1146-1153 (2012).
  • N. Kojima, S. Takeuchi, and Y. Sakai
    "Rapid aggregation of heterogeneous cells and multiple-sized microspheresin methylcellulose medium"
    Biomaterials, 33(18), 4508-4514 (2012).
  • M. Hamon, S. Hanada, T. Fujii, and Y. Sakai
    "Direct Oxygen Supply with Polydimethylsiloxane (PDMS) Membranes Induces a Spontaneous Organization of Thick Heterogeneous Liver Tissues from Rat Fetal Liver Cells in Vitro"
    Cell Transplantation, 21(2-3), 401-410 (2012).
  • H. Matsui, S. Takeuchi, T. Osada, T. Fujii, and Y. Sakai
    "Enhanced bile canaliculi formation enabling direct recovery of biliary metabolites of hepatocytes in 3D collagen gel microcavities"
    Lab on-a-Chip, 33(18), 4508-4514 (2012).
  • [ 2011 ]
  • S. Ostrovidov, Y. Sakai, and T. Fujii
    "Integration of a pump and an electrical sensor into a membrane-based PDMS microbioreactor for cell culture and drug testing"
    Biomed. Microdev., 13(5), 847-864 (2011).
  • K. Montagne, H. Huang, K. Ohara, K. Matsumoto, A. Mizuno, K. Ohta, and Y. Sakai
    "Use of liposome encapsulated hemoglobin as an oxygen carrier for fetal and adult rat liver cell culture"
    J. Biosci. Bioeng, 112(5), 485-490 (2011).
  • T Niino, D Hamajima, K Montagne, S Oizumi, H Naruke, H Huang, Y Sakai, HKinoshita, and T Fujii
    "Laser sintering fabrication of three-dimensional tissue engineering scaffolds with a flow channel network"
    Biofabrication, 3, 034104 (2011).
  • T. Kurakazu, K. Kuribayashi-Shigetomi, Y. T. Matsunaga, H. Kimura, T. Fujii, Y.Sakai, and S. Takeuchi
    "Selective Retrieval Method Using Electrolytically Generated Bubbles for Cell Array Applications"
    Sensors & Actuators: B. Chemical, 159(1), 229-233 (2011).
  • M. Hamon, K. Montagne, N. Kojima, T. Ozawa, R. Ishii, S. Yamaguchi, T. Nagamune, T. Ushida, and Y. Sakai,
    "Avidin-biotin-based approach to forming heterotypic cell clusters and cell sheets on a gas-permeable membrane"
    Biofabrication, 3(3), 034111 (2011).
  • D. A. Ouattara, S.-H. Choi, Y. Sakai, A. R. R. Pérya, and C. Brochot
    "Kinetic modelling of in vitro cell-based assays to characterize non-specific bindings and ADME processes in a static and a perfused fluidic system"
    Toxicol. Lett., 205(3), 310-319 (2011).
  • N. Kojima, S. Takeuchi, and Y. Sakai
    "Establishment of self-organization system in rapidly formed multicellular heterospheroids"
    Biomaterials, 32(26), 6059–6067 (2011).
  • Ginet P, Montagne K, Akiyama S, Rajabpour A, Taniguchi A, Fujii T, Sakai Y, Kim B, Fourmy D, Volz S.
    "Towards single cell heat shock response by accurate control on thermal confinement with an on-chip microwire electrode"
    Lab on-a-Chip, 11(8):1513-20 (2011).
  • Yosuke Nakao, Hiroshi Kimura, Yasuyuki Sakai, and Teruo Fujii
    "Bile canaliculi formation by aligning rat primary hepatocyte in a microfluidic device"
    Biomicrofluidics, 5, 022212 (2011).
  • K.Montagne, R.Plasson, Y.Sakai, T.Fujii, and Y.Rondelez
    "Programming an in vitro DNA oscillator using a molecular networking strategy"
    Mol Syst Biol., 7, (Feb 1;7:466 2011).
  • N. Yamamoto, K. Komori, K. Montagne, H. Matsui, H. Nakayama, S. Takeuchi, and Y. Sakai
    "Cytotoxicity evaluation of reactive metabolites using rat liver homogenate microsome-encapsulated alginate gel microbeads"
    J. Biosci.Bioeng., 111(4), 454-458 (2011).
  • F. Evenou, S. Couderc, B. Kim, T. Fujii, and Y. Sakai
    "Microfibrillated Cellulose Sheets Coating Oxygen-Permeable PDMS Membranes Induce Rat Hepatocytes 3D Aggregation into Stably-Attached 3D Hemispheroids"
    J. Biomater. Sci. Polym. Ed., 22, 1509-1522 (2011).

  • [ 2010 ]

  • M. M. Chowdhury, T. Katsuda, K. Montagne, H. Kimura, N. Kojima, H. Akutsu, T. Ochiya, T. Fujii, and Y. Sakai
    "Enhanced Effects of Secreted Soluble Factor Preserve Better Pluripotent State of Embryonic Stem Cell Culture in a Membrane-based Compartmentalized Micro-bioreactor"
    Biomed. Microdev., 12, 1097-1105 (2010).
  • T. Katsuda, T. Teratani, T. Ochiya, and Y. Sakai
    "Transplantation of a Fetal Liver Cell-loaded Hyaluronic Acid Sponge onto the Mesentery Recovers a Wilson's Disease Model Rat"
    J. Biochem., 148 (3), 281-288 (2010).
  • H. Kimura, H. Takeyama, K. Komori, T. Yamamoto, Y. Sakai, and T. Fujii
    "Microfluidic Device with Integrated Glucose Sensor for Cell-based Assay in Toxicology"
    J. Robotics Mech., 22, 594-600 (2010).
  • N. Kojima, K. Miura, T. Matsuo, H. Nakayama, K. Komori, S. Takeuchi, and Y. Sakai
    "Rapid and Direct Cell-to-Cell Attachment Using Avidin-Biotin Binding System: Large Aggregate Formation in Suspension Culture and Small Tissue Element Formation Having a Precise Microstructure Using Optical Tweezers"
    J. Robotics Mech., 22, 619-622 (2010).
  • Q. Rui, K. Komori, Y. Tian, H. Liu, Y. Luo, and Y. Sakai
    "Electrochemical Biosensor for Detection of H2O2 from Living Cancer Cells Based on ZnO Nanosheets"
    Anal. Chim. Acta, 670, 57-62 (2010).
  • N. Pereira-Rodrigues, P. E. Poleni, D. Guimard, Y. Arakawa, Y. Sakai, and T. Fujii
    "Modulation of Hepatocarcinoma Cell Morphology and Activity by Parylene-C Coating on PDMS"
    PloS One, 5, e9667 (2010).
  • F. Evenou, T. Fujii, and Y. Sakai
    "Spontaneous Formation of Stasbly-attached and 3D-organized Hepatocyte Aggregates on Oxygen-permeable Polydimethylsiloxiane Membrane Having 3D Microstrucctures"
    Biomedical Microdevices, 12, 465-475 (2010).
  • F. Evenou, T. Fujii, and Y. Sakai
    "Spontaneous Formation of Highly Functional Three-dimensional Multilayer from Human Hepatoma Hep G2 Cells Cultured on an Oxygen-permeable Polydimethylsiloxane Membrane"
    Tissue Engineering Part C: Methods, 16, 311-318 (2010).
  • H. Kimura, H. Nakamura, T. Akai, T. Yamamoto, H. Hattori, Y. Sakai, and T. Fujii
    "Study of On-chip Individual Embryo Coculture with Microporous Membrane Supported Endometrial Cells"
    IEEE Trans. NanoBiosci., 8(4), 318-324 (2010).

  • [ 2009 ]

  • K. Montagne, K. Komori, F. Yang, T. Tatsuma, T. Fujii, and Y. Sakai
    "A Micropatterned Cell Array with an Integrated Oxygen-sensitive Fluorescent Membrane"
    Photochemistry & Photobiological Sciences, 8, 1529-1533 (2009).
  • K. Komori, J. Nada, M. Nishikawa, H. Notsu, T. Tatsuma, and Y. Sakai
    "Simultaneous Evaluation of Toxicities Using a Mammalian Cell Array Chip Prepared by Photocatalytic Lithography"
    Analytica Chimica Acta, 653, 222-227 (2009).
  • K. Komori, S. Miyajima, T. Tsuru, T. Fujii, S. Mohri, Y. Ono, and Y. Sakai
    "A Rapid and Simple Evaluation System for Gas Toxicity Using Luminous Bacteria Entrapped by a Polyion Complex Membrane"
    Chemosphere, 77, 1106-1112 (2009).
  • K. Komori, S. Takamiya, N. Kojima, O. Kuwazuru, N. Yoshikawa, K. Furukawa, K. Miyamoto, and Y. Sakai
    "Initial Characterization of a Fibroblast-loaded Porous Elastin Film Reconstituted by a Novel Cross-lonker, Dode-DSP"
    Chemistry Letters, 38, 878-879 (2009).
  • C. Provin, K. Takano, T. Yoshida, Y. Sakai, T. Fujii, and R. Shirakashi
    "Low O2 metabolism of Hep G2 cells cultured at high cell density in a 3D microstructured scaffolds"
    Biomedical Microdevices, 11(2), 485-494 (2009).

  • [ 2008 ]

  • K. Ishii, Y. Kikukawa, M. Shiine, N. Kobayashi, T. Tsuru, Y. Sakai, and A. Sakoda
    "Synthesis and Photophysical Properties of Silica-Gel-Supported Photofunctional (Phthalocyaninato)silicon Complexes"
    European Journal of Inorganic Chemistry, 2975-2981 (2008).
  • V. Senez, E. Lennon, S. Ostrovidov, T. Yamamoto, H. Fujita, Y. Sakai, and T. Fujii
    "Integrated 3-D Silicon Electrodes for Electrochemical Sensing in Microfluidic Environments: Application to Single-Cell Characterization"
    IEEE Sensors Journal, 8, 548-557 (2008).
  • R. Shoji, H. Nakayama, Y. Sakai, S. Mohri, and M. Yamada
    "Evaluation of the ecotoxicity of solid wastes using rapid leaching test and bioassays"
    J. Envron. Sci. Health Part A, 43, 1048-1053 (2008).
  • R. Shoji, S. Mohri, Y. Sakai, and M. Yamada
    "Ecotoxicity Assessment of Sludge and Leaching Test Elutes of Sludge"
    J. Envron. Sci. Health Part A, 43, 1042-1047 (2008).
  • K. Komori, K. Murai, S. Miyajima, T. Fujii, S. Mohri, Y. Ono, and Y. Sakai
    "Development of an in vitro Batch Type-Closed Gas Exposure Device with an Alveolar Epithelial Cell Line, A549, for Toxicity Evaluations of Gaseous Compounds"
    Analytical Sciences, 24, 957-962 (2008) [Hot article and on the cover of the issue].
  • H. Kimura, H. Sakai, T. Yamamoto, Y. Sakai, and T. Fujii
    "An Integrated Microfluidic System for Long-term Perfusion Culture and On-line Monitoring of Intestional Tissue Models"
    Lab on a Chip, 8, 741-746 (2008).
  • C. Provin, K. Takano, Y. Sakai, T. Fujii, and R. Shirakashi
    "A Method for the Design of 3D Scaffolds for High-density Cell Attachment and Determination of Optimun Perfusion culture conditions"
    Journal of Biomechanics, 41, 1436-1449 (2008).
  • N. P. Rodigues, Y. Sakai, and T. Fujii
    "Cell-Based Microfluidic Biochip for the Electrochemical Real-Time Monitoring of Glucose and Oxygen"
    Sensors & Actuators: B, Chemical, 132, 608-613 (2008).
  • S. Hanada, N. Kojima, and Y. Sakai
    "Soluble Factor-Dependent in vitro Growth and Maturation of Rat Fetal Liver Cells in a Three-Dimensional Culture System"
    Tissue Engineeing, 14, 149-160 (2008).
  • M. Nishikawa, T. Yamamoto, N. Kojima, K. Komori, T. Fujii, and Y. Sakai
    "Stable Immobilization of Rat Hepatocytes as Hemispheroids onto Collagen-conjugated Poly-dimethylsiloxane (PDMS) Surfaces: Importance of Direct Oxygenation Through PDMS for Both Formation and Function"
    Biotechnol. Bioeng., 99, 1472-2481 (2008).
  • M. Nishikawa, N. Kojima, K. Komori, T. Yamamoto, T. Fujii, and Y. Sakai
    "Enhanced Maintenance and Functions of Rat Hepatocytes Induced by Combination of On-Site Oxygenation and Coculture with Fibroblasts"
    Journal of Biotechnology, 133, 253-260 (2008).

  • [ 2000 - 2007 ]

  • H. Naruto, H. Huang, M. Nishikawa, N. Kojima, A. Mizuno, K. Ohta, and Y. Sakai
    "Feasibility of Direct Oxygenation of Primary-Cultured Rat Hepatocytes Using Polyethylene Glycol Decorated Liposome-Encapsulated Hemoglobin (LEH)"
    Journal of Bioscience and Bioengineering, 104, 343-346 (2007).
  • H. Kimura, M. Nishikawa, T. Yamamoto, Y. Sakai, and T. Fujii
    "Microfluidic Perfusion Culture of Human Hepatocarcinoma"
    Journal of Robotics and Mechatronics, 19, 550-556 (2007).
  • H. Nakayama, H. Kimura, K. Komori, T. Fujii, and Y. Sakai
    "Development of a Multi-Compartment Micro-Cell Culture Device as a Future on-Chip Human: Fabrication of a Three-Compartment Device and Immobilization of Rat Mature Adipocytes for the Evaluation of Chemical Distributions"
    Journal of Robotics and Mechatronics, 19, 544-549 (2007).
  • H. Huang, S. Oizumi, N. Kojima, T. Niino, and Y. Sakai
    "Avidin-Biotin Binding-Based Cell Seeding and Perfusion Culture of Liver-Derived Cells in a Porous Scaffold with a Three-Dimensional Interconnected Flow-Channel Network"
    Biomaterials, 28, 3815-3823 (2007).
  • N. Kojima, T. Matsuo, and Y. Sakai
    "Rapid Hepatic Cell Attachment onto Biodegradable Polymer Surfaces without Toxicity Using an Avidin-Biotin Binding System"
    Biomaterials, 27(28), 4904-4910 (2006).
  • H. Huang, S. Hanada, N. Kojima, and Y. Sakai
    "Enhanced Functional Maturation of Fetal Porcine Hepatocytes in Three-dimensional Poly-L-lactic Acid Scaffolds: A Culture Condition Suitable for Engineered Liver Tissues in Large-Scale Animal Studies"
    Cell Transplantation, 15(8-9), 799-809 (2006).
  • Y. Cho, T. Yamamoto, Y. Sakai, T. Fujii, and B. Kim
    "Development of microfluidic device for electrical/physical characterization of single cell"
    J. Microelectromechanical systems, 15(2), 287-295 (2006).
  • M. Sagehashi, T. Fukuda, T. Fujii, Y. Sakai and A. Sakoda
    "Elution and adsorptive concentration of Japanese cedar (Cryptomeria japonica) pollen allergen in environmental water"
    Water Science & Technology, 52(8), 37-43 (2005).
  • I. Shitanda, K. Takada, Y. Sakai, and T. Tatsuma
    "Amperometric Biosensing Systems Based on Motility and Gravitaxis of Flagellate Algae for Aquatic Risk Assessment"
    Analytical Chemistry, 77(20), 6715-6718 (2005).
  • Y. Sakai, K. Tomita, M. Suzuki, Y. Ono, and A. Sakoda
    "Development of a toxicity evaluation system for gaseous compounds using air-liquid interface culture of a human bronchial epithelial cell line, Calu-3"
    AATEX, 11(1), 59-67 (2005).
  • N. Kojima, N. Shiojiri, Y. Sakai, and A. Miyajima
    "Expression of neuritin during liver maturation and regeneration"
    FEBS Letters, 579(21), 4562-4566 (2005).
  • S. Okajima, T. Yamaguchi, Y. Sakai, and S. Nakao
    "Regulation of cell adhesion using a signal-responsive membrane substrate"
    Biotechnology and Bioengineering, 91(2), 237-243 (2005).
  • K. Naruse, H. Nagashima, Y. Sakai, N. Kokudo, and M. Makuuchi
    "Development and Perspectives of Perfusion Treatment for Liver Failure"
    Surgery Today, 35(7), 507-517 (2005).
  • S. Okajima, Y. Sakai, and T. Yamaguchi
    "Development of a regenerable cell culture system that senses and releases dead cells"
    Langmuir, 21(9), 4043-4049 (2005).
  • I. Shitanda, K. Takada, Y. Sakai, and T. Tatsuma
    "Compact amperometric algal biosensors for the evaluation of water toxicity"
    Analytica Chimica Acta, 530(2), 191-197 (2005).
  • K. Shimizu, O. Endo, S. Goto, A. Sakoda, Y. Ono, and Y. Sakai
    "Bioassay-based evaluation of toxicity of suspended particulate matter in humans: integrated uses of alveolar cells (A549) in air-liquid interface culture and hepatocarcinoma cells (Hep G2)"
    Biochemical Engineering Journal, 22(1), 1-9 (2004).
  • H. Shindoh, K. Naruse, Y. Sakai, and M. Makuuchi
    "Efficacy of immunoadsorbent devices for maintaining hepatic function in ex vivo xenogenic direct hemoperfusion"
    Int. J. Artif. Organs, 27(4), 294-302 (2004).
  • J. Jiang, N. Kojima, L. Guo, K. Naruse, M. Makuuchi, A. Miyajima, W. Yan, and Y. Sakai
    "Efficacy of Engineered Liver Tissue Based on Poly-L-lactic Acid Scaffolds and Fetal Mouse Liver Cells Cultured with Oncostatin M, Nicotinamide, and Dimethyl Sulfoxide"
    Tissue Engineering, 10(9/10), 1577-1586 (2004).
  • S. Ostrovidov, J. Jiang, Y. Sakai, and T. Fujii
    "Membrane-Based PDMS Microbioreactor for Perfused 3D Primary Rat Hepatocyte Cultures"
    Biomedical Microdevices, 6(4), 279-287 (2004).
  • E. Leclerc, K. S. Furukawa, F. Miyata, Y. Sakai, T. Ushida, and T. Fujii
    "Fabrication of microstructures in photosensitive biodegradable polymers for tissue engineering applications"
    Biomaterials, 25, 4683-4690 (2004)
  • Y. Sakai, M. Otsuka, J. Kozasa, F. Miyata, K. S. Furukawa, and A. Yamashita
    "Ultraviolet-irradiation-based photofabrication that simultaneously produces a macroporous structure and flow channels using a photoreactive biodegradable polymer and a gas-forming azoamide compound"
    Biochemical Engineering Journal, 20(2-3), 163-171 (2004).
  • E. Leclerc, Y. Sakai, and T. Fujii
    "Perfusion Culture of Fetal Human Hepatocytes in Microfluidic-Environments"
    Biochemical Engineering Journal, 20, 143-148 (2004).
  • Y. Sakai, M. Otsuka, S. Hanada, Y. Nishiyama, Y. Konishi, and A. Yamashita
    "A novel poly-L-lactic acid scaffold that possesses a macroporous structure and a branching/joining three-dimensional flow channel network: Its fabrication and application to perfusion culture of human hepatoma Hep G2 cells"
    Materials Science & Engineering, C: Biomimetic and Supramolecular Systems, 24(3), 379-386 (2004).
  • J. Jiang, N. Kojima, T. Kinoshita, A. Miyajima, W. Yan, and Y. Sakai
    "Cultivation and induction of fetal liver cells in poly-L-lactic acid scaffold"
    Materials Science & Engineering, C: Biomimetic and Supramolecular Systems, C24(3), 361-363 (2004).
  • E. Leclerc, F. Miyata, K. S. Furukawa, T. Ushida, Y. Sakai, and T. Fujii
    "Effect on liver cells of stepwise microstructures fabricated in a photosensitive biodegradable polymer by softlithography"
    Materials Science & Engineering, C: Biomimetic and Supramolecular Systems, C24(3), 349-354 (2004).
  • S.-H. Choi, O. Fukuda, A. Sakoda, and Y. Sakai
    "Enhanced cytochrome P450 capacities of Caco-2 and Hep G2 cells in new coculture system under the static and perfused conditions: evidence for possible organ-to-organ interactions against exogenous stimuli"
    Materials Science & Engineering, C: Biomimetic and Supramolecular Systems, C24(3), 333-339 (2004).
  • S.-H. Choi, M. Nishikawa, A. Sakoda, and Y. Sakai
    "Feasibility of a simple double-layered coculture system incorporating metabolic processes of the intestine and liver tissue: application to the analysis of benzo[a]pyrene toxicity"
    Toxicology in Vitro, 18(3), 393-402 (2004).
  • E. Leclerc, Y. Sakai, and T. Fujii
    "Microfluidic PDMS (Polydimethylsiloxane) Bioreactor for Large-Scale Culture of Hepatocytes"
    Biotechnology Progress, 20(3), 750-755 (2004).
  • S. Hanada, H. Kayano, J. Jiang, N. Kojima, A. Miyajima, A. Sakoda, and Y. Sakai
    "Enhanced in vitro maturation of subcultivated fetal human hepatocytes in three dimensional culture using poly-L-lactic acid scaffolds in the presence of oncostatin M"
    Int. J. Artif. Organs., 26(10), 943-951 (2003).
  • Y. Sakai, O. Fukuda, S.-H. Choi, and A. Sakoda
    "Development of a biohybrid simulator for absorption and biotransformation processes in humans based on in vitro models of the small intestine and the liver tissues"
    J. Artif. Organs, 6,273-281 (2003).
  • K Naruse, Y.Sakai, L. Guo, T. Natori, J. Shindo, Y. Karasawa, Y. Iida, K. Kojima, K. Michishita, and M. Makuuchi
    "Development of a new extracorporeal whole-liver perfusion system"
    J. Artif. Organs, 6, 211-217 (2003).
  • K. Naruse, Y.Sakai, T. Natori, L. Guo, J. Shindo, Y. Iida, K. Michishita, Y. Karasawa, K. Kojima, and M. Makuuchi
    "Xenogeneic direct hemoperfusion using whole swine liver for liver failure in dogs"
    J. Surg. Res., 111, 229-235 (2003).
  • K. S. Furukawa, H. Suenga, K. Toita, T. Ushida, Y. Sakai, and T. Tateish
    "Rapid and large scale formation of chondrocyte aggregates by rotational culture"
    Cell Transplant.,12, 475-479 (2003).
  • E. Leclerc, Y. Sakai, and T. Fujii
    "Cell culture in 3-dimensional microfluidic structure of PDMS (polydimethylsiloxane)"
    Biomedical Microdevices, 5(2), 109-114 (2003).
  • K. Naruse, Y. Sakai, L. Guo, J. Shindoh, J.-C. Son, and M. Makuuchi
    "Development of a xenogeneic direct hemoperfusion method in a bioartificial liver system"
    J. Artif. Organs., 5, 257-264 (2002).
  • B-S. Kim, H. Fujita, Y. Sakai, A. Sakoda, and M. Suzuki
    "Catalitic ozonation of an organophophorous pesticide using microporous silicate and its effect on total toxicity reduction"
    Water Sci. Technol., 46(4-5), 35-41 (2002).
  • K. Furukawa, T. Ushida, K. Toita, Y. Sakai, and T. Tateishi
    "Hybrid of gel-cultured smooth muscle cells with PLLA sponge as a scaffold towards blood vessel regeneration"
    Cell Transplant., 11(5), 475-480 (2002).
  • J. Jiang, N. Kojima, T. Kinoshita, A. Miyajima, W. Yan, and Y. Sakai
    "Cultivation of fetal mouse liver cells in a three-dimensional poly-L-lactic acid scaffold in the presence of oncostatin M"
    Cell Transplant.,11(5), 403-406 (2002).
  • Y. Sakai, J. Jiang, N. Kojima, T. Kinoshita, and A. Miyajima
    "Enhanced in vitro maturation of fetal liver cells with oncostatin M, nicotinamide and dimethylsulfoxide"
    Cell Transplant., 11(5), 435-441 (2002).
  • A. Kamiya, N. Kojima, T. Kinoshita, Y. Sakai, and A. Miyaijma
    "Maturation of fetal hepatocytes in vitro by extracellular matrices and oncostatin M: induction of tryptophan oxygenase"
    Hepatology, 35(6), 1351-1359 (2002).
  • K. Furukawa, T. Ushida, K. Kunii, Y. Sakai, J. Tanaka, and T. Tateishi
    "Effects of hormone and growth factor on formation of fibroblast-aggregates for tissue-engineered skin"
    Materials Science & Engineering, C: Biomimetic and Supramolecular Systems, C17(1-2), 59-62 (2001).
  • K. Naruse, Y. Sakai, L. Guo, Y. Sakamoto, T. Kobayashi, C. Puliatti, G. Aronica, W. Morale, F. Leone, S. Qiang, S.-G. Ming, S. Ming, Z. Li, S.-J. Chang, M. Suzuki, and M. Makuuchi
    "Efficacy of nonwoven fabric bioreactor immobilized with porcine hepatocytes for ex vivo xenogeneic perfusion treatment of liver failure in dogs"
    Artificial Organs, 25(4), 273-280 (2001).
  • Y. Sakai, R. Shoji, B.-S. Kim, A. Sakoda, and M. Suzuki
    "Cultured human-cell-based bioassays for environmental risk management"
    Environ. Monit. Assess., 70, 57-70 (2001).
  • K. Furukawa, T. Ushida, Y. Sakai, K. Kunii, J. Tanaka, and T. Tateishi
    "Tissue-engineered skin using aggregates of normal human skin fibroblasts and biodegradable material"
    J. Artif. Organs, 4, 353-356 (2001).
  • K. S. Furukawa, T. Ushida, Y. Sakai, M. Suzuki, and T. Tateishi
    "Formation of human fibroblasts-aggregates (spheroids) by rotational culture for tissue-engineered skin"
    Cell Transplant.,10, 441-445 (2001).
  • Y. Sakai, K. Furukawa, T. Ushida, T. Tateishi, and M. Suzuki
    "In vitro organization of biohybrid rat liver tissue incorporating growth-factor- and hormone-releasing biodegradable-polymer microcapsules"
    Cell Transplant., 10, 479-483 (2001).
  • Y. Sakai, T. Arai, A. Sakoda, and M. Suzuki
    "Development of a simple double-layered cell culture system using Caco-2 and TIG-1 cells as a new cytotoxicity test"
    AATEX, 7(2-3), 47-58 (2001).
  • R. Shoji, A. Sakoda, Y. Sakai, H. Utsumi, and M. Suzuki
    "Formulating bioassay data of chemicals and environmental water"
    Water Sci. and Technol., 42(3-4), 115-124 (2000).
  • Mantalaris, A., N. Panoskaltsis, Y. Sakai, P. Bourne, C. S. Chang, E. M. Messing, and J.H.D. Wu
    "Localization of androgen receptor expression in human bone marrow"
    J. Pathol., 192(5), 361-366, (2000).
  • R. Shoji, A. Sakoda, Y. Sakai, H. Utsumi, and M. Suzuki
    "A new assay for hepatotoxicity using LDL-uptaking activity of liver cells"
    J. Health Sci., 46(6), 493-502 (2000).
  • R. Shoji, Y. Sakai, A. Sakoda, and M. Suzuki
    "Development of a rapid and sensitive bioassay device using human cells immobilized in macroporous microcarriers for the on-site evaluation of environmental waters"
    Appl. Microb. Biotechnol., 54, 432-438 (2000).
  • Y. Sakai, R. Shoji, Y. Mishima, A. Sakoda, and M. Suzuki
    "Rapid and sensitive neurotoxicity test based on the morphological changes of PC12 cells with simple computer-assisted image analysis"
    Journal of Bioscience and Bioengineering, 90(1), 20-24 (2000).
  • R. Shoji, Y. Sakai, A. Sakoda, and M. Suzuki
    "Preservation of microplate-attached human hepatoma cells and their use in cytotoxicity tests"
    Cytotechnology, 32(2), 147-155 (2000).

  • [ 1991 - 1999 ]

  • Y. Sakai, K. Naruse, I. Nagashima, T. Muto, and M. Suzuki
    "A new bioartificial liver using porcine hepatocyte spheroids in high-cell-density suspension perfusion culture: in vitro performance in synthesized culture medium and 100% human plasma"
    Cell Transplant., 8, 531-541 (1999).
  • K. Naruse, I. Nagashima, Y. Sakai, G.-X. Jiang, M. Suzuki, T. Muto, and M. Makuuchi
    "Efficacy of a bioreactor filled with porcine hepatocytes immobilized on non-woven fabrics for ex vivo direct hemoperfusion treatment of liver failure in pigs"
    Artif. Organs, 22(12), 1031-1037 (1998).
  • R. Shoji, A. Sakoda, Y. Sakai, M. Suzuki, and H. Utsumi
    "Rapid bioassay of toxicity in environmental water by LDL-uptaking activity of human cell"
    Water Science and Technology, 38(7), 271-278 (1998).
  • A. Sakoda, Y. Sakai, K. Hayakawa, and M. Suzuki
    "Adsorption of viruses in water environment onto solid surfaces"
    Water Science and Technology, 35(7), 107-114 (1997).
  • K. Naruse, Y. Sakai, I. Nagashima, G.-X. Jiang, M. Suzuki, and T. Muto
    "Comparison of porcine hepatocyte spheroids and single hepatocytes in the non-woven fabric bioartificial liver module"
    Int. J. Artif. Organs, 19(10), 605-609 (1996).
  • Y. Sakai, K. Ichikawa, A. Sakoda, and M. Suzuki
    "Quantitative comparison of rat hepatocyte functions in two improved culture systems with or without rat liver epithelial cell line"
    Cytotechnology, 21(3), 243-252 (1996).
  • K. Naruse, Y. Sakai, I. Nagashima, G.-X. Jiang, M. Suzuki, and T. Muto
    "Development of a new bioartificial liver module filled with porcine hepatocytes immobilized on non-woven fabrics"
    Int. J. Artif. Organs, 19(6), 347-352 (1996).
  • K. Ichikawa, Y. Sakai, A. Sakoda, and M. Suzuki
    "Long-term cytotoxicities of various pesticides evaluated by albumin secretion of primary cultured rat hepatocytes"
    Biotechnology Techniques, 10(2), 99-102 (1996).
  • Y. Sakai, K. Naruse, I. Nagashima, T. Muto, and M. Suzuki
    "Large-scale preparation and functions of porcine hepatocyte spheroids"
    Int. J. Artif. Organs, 19(5), 294-301 (1996).
  • Y. Sakai, K. Naruse, I. Nagashima, T. Muto, and M. Suzuki
    "In vitro function of porcine hepatocyte spheroids in 100% human plasma"
    Cell Transplant., 5(5S-1), S41-S43, (1996).
  • Y. Sakai, K. Naruse, I. Nagashima, T. Muto, and M. Suzuki
    "Short-term hypothermic preservation of porcine hepatocyte spheroids using UW solution"
    Cell Transplant., 5(4), 501-511 (1996).
  • Y. Sakai, K. Naruse, I. Nagashima, T. Muto, and M. Suzuki
    "Functional stability of porcine hepatocyte spheroids in various culture systems under 100% porcine and human plasma conditions"
    Artificial Organs, 20(1), 56-60 (1996).
  • Y. Sakai, A. Sakoda, and M. Suzuki
    "Attachment kinetics of animal cells immediately after contact onto specific and/or non-specific surfaces"
    Journal of Chemical Engineering of Japan, 28(5), 590-5 (1995).
  • Y. Sakai and M. Suzuzki
    "Stable immobilization and functional expressions of hepatocyte spheroids rapidly formed by suspension culture"
    Artificial Organs Today, 4, 129-140 (1994).
  • K. Ichikawa, Y. Sakai, A. Sakoda, and M. Suzuki
    "Primary culture of rat hepatocytes using membrane-supported collagen sandwich with hormone-free medium"
    Biotechnology Techniques, 8(6), 385-8 (1994).
  • M. Suzuki, K. Ichikawa, A. Sakoda, and Y. Sakai
    "Long-term culture of primary rat hepatocytes with high albumin secretion using membrane-supported collagen sandwich"
    Cytotechnology, 11, 213-218 (1993).
  • Y. Sakai, K. Furukawa, and M. Suzuki
    "Immobilization and long-term albumin secretion of hepatocyte spheroids rapidly formed by rotational tissue culture methods"
    Biotechnology Techniques, 6(6), 527-32 (1992).
  • Y. Sakai and M. Suzuki
    "Formation of spheroids of adult rat hepatocytes on polylysine-coated surfaces and their albumin production"
    Biotechnology Techniques, 5(4), 299-302 (1991).