国際ジャーナル
in press
Comparative analysis of bile canaliculi formation in freshly isolated and flask-delivered human hepatocytes from humanized mice livers under sufficient oxygen supply
Tokito F, Gong Ya, Kurniawan D A, Kaneko S, Shioda H, Sangho L, Kushima A, Choi H, Inamatsu R, Tateno C, Nishikawa M, Sakai Y.
Fundamental Toxicological Sciences, in press.Gut-Liver Microphysiological Systems Revealed Potential Crosstalk Mechanism Modulating Drug Metabolism
Dhimas Agung Kurniawan, Sylvia Leo, Mutsumi Inamatsu, Sohei Funaoka, Taichi Aihara, Mizuno Aiko, Inoue Rei, Takeshi Sakura, Hiroshi Arakawa, Yukio Kato, Tomoaki Matsugi, Katsuhiro Esashika, Nobuaki Shiraki, Shoen Kume, Kenta Shinha, Hiroshi Kimura, Masaki Nishikawa, Yasuyuki Sakai.
PNAS Nexus, in press.Modulation of Hepatic Tight Junctions via Coculture with Cholangiocytes Enables Non-Destructive Bile Recovery
Tokito F, Kiyofuji M, Choi H, Nishikawa M, Takezawa T, Sakai Y. J. Biosci.
Bioeng., in press.Mechanobiological stimulation in Organ-on-a-Chip systems reduces hepatic drug metabolic capacity in favor of regenerative specialization
Benedikt Scheidecker, Stephane Poulain, Masahiro Sugimoto, Takumi Kawanishi, Soo Hyeon Kim, Hiroshi Arakaw, Yukio Kato, Mathieu Danoy, Masaki Nishikawa, and Yasuyuki Sakai
Biotechnol. Bioeng., in press.
https://doi.org/10.1002/bit.28653A Highly Efficient Cell Culture Method using Oxygen-Permeable PDMS-Based Honeycomb Microwells Produces Functional Liver Organoids from Human Induced Pluripotent Stem Cell-Derived Carboxypeptidase M Liver Progenitor Cells
Tia Utami, Mathieu Danoy, Rubina Rahaman Khadim, Fumiya Tokito, Hiroshi Arakawa,Yukio Kato, Taketomo Kido, Atsushi Miyajima, Masaki Nishikawa, Yasuyuki Sakai
Biotechnol. Bioeng., in press.
https://doi.org/10.1002/bit.286402024
Development of erythrocyte-mimetic PFOB/PDMS thermoplastic elastomer core-shell microparticles via SPG membrane emulsification
Qiming Zhang, Natsuko F. Inagaki, Hiromi Yoshida, Masamichi Kamihira, Yasuyuki Sakai, Taichi Ito
Journal of Membrane Science, 689, 122119(2024).
https://doi.org/10.1016/j.memsci.2023.122119
Injectable phase-separated tetra-armed poly(ethylene glycol) hydrogel scaffold allows sustained release of growth factors to enhance the repair of critical bone defects
Shant Nepal, Jinyan Si, Shohei Ishikawa, Masaki Nishikawa, Yasuyuki Sakai,Aya M. Akimoto, Hiroyuki Okada, Shinsuke Ohba, Ung-il Chung, Takamasa Sakai, Hironori Hojo
Reg. Ther, 25, 24-34 (2024).
https://doi.org/10.1016/j.reth.2023.11.008
2023
Inflammatory liver tissue formation using oxygen permeable membrane based culture platform
Marie Shinohara, Qiao You Lau, Fuad Gandhi Torizal, Hyunjin Choi, Yasuyuki Sakai
J. Biosci. Bioeng., 2023;136(4):327-333.
https://doi.org/10.1016/j.jbiosc.2023.06.013
Optimization of physical microenvironment to maintain the quiescence of human induced pluripotent stem sell-derived hepatic stellate cells
Ya Gong, Mathieu Danoy, Taketomo Kido, Mitsuhashi Kento, Hyunjin Choi, Tomoaki Matsugi, Jingjing Yang, Katsuhiro Esashika, Jun Takahashi, Masaki Nishikawa, Taichi Ito, Atsushi Miyajima, Yasuyuki Sakai
Biotechnol Bioeng. 2023 Aug;120(8):2345-2356.
https://doi.org/10.1002/bit.28485
Transcriptomic and proteomic studies suggest the establishment of advanced zonation‐like profiles in human induced pluripotent stem cell‐derived liver sinusoidal endothelial cells and carboxypeptidase M‐positive liver progenitor cells cocultured in a fluidic microenvironment
Mathieu Danoy, Stéphane Poulain, Rachid Jellali, Benedikt Scheidecker, Yannick Tauran, Marjorie Leduc, Johanna Bruce, Soo Hyeon Kim, Taketomo Kido, Atsushi Miyajima, Yasuyuki Sakai, Eric Leclerc
Hepatology Research, 53, 7(2023).
https://doi.org/10.1111/hepr.13893Generation of β-like cell subtypes from differentiated human induced pluripotent stemcells in 3D spheroids
Lisa Morisseau, Fumiya Tokito, Stephane Poulain, Valerie Plaisance, Valerie Pawlowski,Soo Hyeon Kim, Cecile Legallais, Rachid Jellali, Yasuyuki Sakai, Amar Abderrahmani and Eric Leclerc
Molecular Omics, 9(10):810-822 (2023).
https://doi.org/10.1039/d3mo00050h
Spheroid on-demand printing and drug screening of endothelialized hepatocellular carcinoma model at different stages
Liu T, Zhou C, Ji J, Xu X, Xing Z, Shinohara M, Sakai Y, Sun T, Feng X, Yu Z, Pang Y, Sun W.. Biofabrication. 2023 25; 15(4) (2023).
https://doi.org/10.1088/1758-5090/ace3f9
Particle-tracking-based strategy for the optimization of agitation conditions in a suspension culture of human induced pluripotent stem cells in a shaking vessel
Ikki Horiguchi, Hotaka Nagate, Yasuyuki Sakai
J. Biosci. Bioeng., 135(5), 411-416 (2023).
http://doi.org/10.1016/j.jbiosc.2023.02.007
Dialysis based-culture medium conditioning improved the generation of human induced pluripotent stem cell derived-liver organoid in a high cell density
Fuad Gandhi Torizal, Tia Utami, Lau Qiau You, Kousuke Inamura, Masaki Nishikawa, and Yasuyuki Sakai
Sci. Rept., 12, 20774 (2023).
https://doi.org/10.1038/s41598-022-25325-9
2022
Analysis of the transcriptome and metabolome of pancreatic spheroids derived from human induced pluripotent stem cells and matured in organ-on-chip
Amal Essaouiba, Rachid Jellali, Stéphane Poulain, Fumiya Tokito, Françoise, Gilard, Bertrand Gakière, Soo Hyeon Kim, Cécile Legallais, Yasuyuki Sakai, Eric Leclerc
Mol. Omics, 18, 791-804 (2022).
http://doi.org/10.1039/d2mo00132bDevelopment of a pancreas-liver organ-on-chip coculture model for organ-to-organ interaction studies
Amal Essaouiba, Teru Okitsu, Rie Kinoshita, Rachid Jellali, Marie Shinohara, Mathieu Danoy, Cécile Legallais, Yasuyuki Sakai, Eric Leclerc
Biochemical Engineering Journal, 164, 107783 (2022).
https://doi.org/10.1016/j.bej.2020.107783A novel HGF/SF receptor (MET) agonist promotes differentiation of human pluripotent stem cells into hepatocyte-like cells
Tauran, Yannick; Lereau Bernier, Myriam; Segard, Bertrand David; Danoy, Mathieu; Kimura, Keiichi; Shinohara, Marie; Brioude, Arnaud; Sakai, Yasuyuki; Dejonge, Hugo; Melnik, Oleg; Vicogne, Jerome; Leclerc, Eric
Dev. Growth Differ., 64(9), 527-536 (2022).
http://doi.org/10.1111/dgd.12818
Simulation of the crosstalk between glucose- and acetaminophen metabolism in a liver zonation model.
Maeda, K.; Hagimori, S.; Sugimoto, M.; Sakai, Y.; Nishikawa, M.
Front. Pharmacol., 13:995597.
https://doi.org/10.3389/fphar.2022.995597
Cryopreserved human hepatocytes culture optimization on polymethylpentene oxygen permeable membranes for drug screening purposes.
Mathieu Danoy, Benedikt Scheidecker, Hiroshi Arakawa, Katsuhiro
Esashika, Naoki Ishida, Hiroyasu Ito, Hisaaki Yanai, Jun Takahashi,
Masaki Nishikawa, Yukio Kato, Yasuyuki Sakai
Fundamental Toxicological Sciences, 9(4), 135-144 (2022).
https://doi.org/10.2131/fts.9.135
Computational simulation of liver fibrosis dynamics
Misa Yoshizawa, Masahiro Sugimoto, Minoru Tanaka, Yusuyuki Sakai, Masaki
Nishikawa
Sci. Rept., 12, 14112 (2022).
https://doi.org/10.1038/s41598-022-18123-w
Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs
Rubina Rahaman Khadim, Raja Kumar Vadivelu, Tia Utami, Fuad Gandhi Torizal, Masaki Nishikawa and Yasuyuki Sakai
International Journal of Molecular Sciences, 23(13):7272 (2022).
https://doi.org/10.3390/ijms23137272
Scarable Formation of HepG2 Spheroids in an Oxygen-Permeable Microwell Device to Mimic Early-Stage Hepatocellular Carcinomas for Anti-Tumor Drug Evaluation
Jianyu He, Chang Zhou, Xiaolei Xu, Zhou zhenzhen, Mathieu Danoy, Marie Shinohara, Wenjin Xiao, Xiaobin Feng, Yilei Mao, Wei Sun, Yasuyuki Sakai, Huayu Yang, Yuan Pang
Advanced healthcare Materials, 11(18), 2200863 (2022).
https://doi.org/10.1002/adhm.202200863
Real-time High-resolution Measurement of Pancreatic β Cell Electrophysiology Based on Transparent Thin-film Transistor Microelectrode Arrays
Dongchen Zhu, Anne-Claire Eiler, Satoshi Ihida, Yasuyuki Sakai, Hiroshi Toshiyoshi, Agnes Tixier-Mita, Kikuo Komori
IEEJ Transactions on Sensors and Micromachines, 2022, Volume 142, Issue 10, Pages 266-272.
https://doi.org/10.1541/ieejsmas.142.266
Accurate evaluation of hepatocyte metabolisms on a noble oxygen-permeable material with low sorption characteristics
Masaki Nishikawa, Hiroyasu Ito, Fumiya Tokito, Keita Hirono, Kousuke Inamura, Benedikt Scheidecker, Mathieu Danoy, Takumi Kawanishi, Hirohsi Arakawa, Yukio Kato, Katsuhiro Esashika, Hiroshi Miyasako, Yasuyuki Sakai
Frontiers in Toxicology, 4:810478 (2022).
https://doi.org/10.3389/ftox.2022.810478
Production of homogenous size-controlled human induced pluripotent stem cell aggregates using ring-shaped culture vessel
Fuad Gandhi Torizal, Seong Min Kim, Ikki Horiguchi, Kousuke Inamura, Ikumi Suzuki, Takashi Morimura, Masaki Nishikawa, Yasuyuki Sakai
J. Tissue Eng. Regen. Med., March 2022, Vol. 16, issue 3, page 254-266
https://doi.org/10.1002/term.3278
Influence of CPM-dependent sorting on the multi-omics profile of hepatocyte-like cells matured in microscale biochips
Danoy Mathieu, Poulain Stephane, Scheidecker Benedikt, Jellali Rachid, Tauran Yannick, Leduc Marjorie, Bruce Johanna, Gilard Francoise, Gakiere Bertrand, Arakawa Hiroshi, Kato Yukio, Kim Soo Hyeon, Kido Taketomo, Miyajima Atsushi, Sakai Yasuyuki, Leclerc Eric.
Biochemical Engineering Journal, 2022, 108408, ISSN 1369-703X.
https://doi.org/10.1016/j.bej.2022.108408
2021
A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation
Fuad Gandhi Torizal, Qiao You Lau, Masato Ibuki, Yoshikazu Kawai, Masato Horikawa, Masataka Minami, Tatsuo Michiue, Ikki Horiguchi, Masaki Nishikawa & Yasuyuki Sakai
Communications Biology volume 4, Article number: 1316 (2021)
https://doi.org/10.1038/s42003-021-02848-x
Development of human iPSC-derived quiescent hepatic stellate cells for drug discovery and in vitro disease modeling
Yuta Koui, Misao Himeno, Yusuke Mori, Natsuki Maeda, Luyao Wang, Yoshiko Kamiya, Hiroko Anzai, Tadanori Yamada, Yasuyuki Sakai, Atsushi Miyajima, Taketomo Kido
Stem Cell Reports, Vol. 16 1-14, December 14, 2021
https://doi.org/10.1016/j.stemcr.2021.11.002
Bioinspired perfluorocarbon-based oxygen carriers with concave shape and deformable shell
Xiaoting Fu, Seiichi Ohta, Takahiro Kawakatsu, Masamichi Kamihira, Yasuyuki Sakai, Taichi Ito
Advanced Materials Technologies, 2021, 2100573.
https://doi.org/10.1002/admt.202100573
In Vitro Enzymatic Electrochemical Monitoring of 1 Glucose Metabolism and Production in Rat Primary Hepatocytes on Highly O2 Permeable Plates
Kikuo Komori, Masataka Usui, Kohei Hatano, Yuma Hori, Keita Hironou, Dongchen
Zhu, Fumiya Tokito, Masaki Nishikawa, Yasuyuki Sakai and Hiroshi
Kimura
Bioelectrochemistry, 143, 107972 (2021).
2021 Oct 7;143:107972.
https://doi.org/10.1016/j.bioelechem.2021.107972
Toward the development of a label-free multiple immunosensor based on thin film transistor microelectrode arrays
Dongchen Zhu, Grant A. Cathcart, Satoshi Ihida, Hiroshi Toshiyoshi, Agnès Tixier-Mita,
Yasuyuki Sakai, and Kikuo Komori
Journal of Micromechanics and Microengineering, 31, 115002 (2021).
https://doi.org/10.1088/1361-6439/ac2547
A Kinetic‐Pump Integrated Microfluidic Plate (KIM‐Plate) with High Usability for Cell Culture‐based Multi‐Organ Microphysiological Systems
Kenta Shinha, Wataru Nihei, Hiroko Nakamura, Tomomi Goto, Takumi Kawanishi, Naoki Ishida, Nao Yamazaki, Yuki Imakura, Shinji Mima, Kosuke Inamura, Hiroshi Arakawa, Masaki Nishikawa, Yukio Kato, Yasuyuki Sakai, Hiroshi Kimura
Micromecanics, 12(9), 1007 (2021).
https://doi.org/10.3390/mi12091007
Differentiation of human induced pluripotent stem cell-derived endocrine progenitors to islet-like cells using dialysis suspension culture system
Hyunjin CHOI, Marie SHINOHARA, Masato IBUKI, Masaki NISHIKAWA, Yasuyuki SAKAI
Cellis, 10(8), (2021).
https://doi.org/10.3390/cells10082017
Characterization of the proteome and metabolome of human liver sinusoidal endothelial-like cells derived from induced pluripotent stem cells
Mathieu Danoy, Rachid Jellali, Yannick Tauran, Johanna Bruce, Marjorie Leduc, Françoise Gilard, Bertrand Gaki`ere, Benedikt Scheidecker, Taketomo Kido, Atsushi Miyajima, Fabrice Soncin, Yasuyuki Sakai, Eric Leclerc
Differentiation 120 (2021) 28–35.
https://doi.org/10.1016/j.diff.2021.06.001
Investigation of the hepatic development in the coculture of hiPSCs-derived LSECs and HLCs in a fluidic microenvironment
Mathieu Danoy, Yannick Tauran, Stephane Poulain, Rachid Jellali, Johanna Bruce, Marjorie Leduc, Morgane Le Gall, Yuta Koui, Hiroshi Arakawa, Francoise Gilard, Bertrand Gakiere, Yukio Kato, Charles Plessy, Taketomo Kido, Atsushi Miyajima, Yasuyuki Sakai, Eric
Leclerc
APL Bioengineering 5, 026104 (2021).
https://doi.org/10.1063/5.0041227
Prospect of in vitro bile fluids collection in improving cell based assay of liver function
Astia Rizki Safitri, Fumiya Tokito, Masaki Nishikawa, Minoru Tanaka, Kazuya Maeda, Hiroyuki Kusuhara and Yasuyuki Sakai
Front. Toxicol.,03 june 2021.
https://doi.org/10.3389/ftox.2021.657432
"Fabrication of a porous three-dimensional scaffold with inter-connected
flow channels: co-cultured liver cells and in-vitro haemocompatibility
assessment"
Muxin Li, Rubina Rahaman Khadim *, Mitsuru Nagayama, Marie, Shinohara,
Kousuke Inamura, Mathieu Danoy, Masaki Nishikawa, Katsu Furukawa, Yasuyuki
Sakai, Toshiki Niino
Appl. Sci. 2021, 11(6), 2473.
*Equal contribution
https://doi.org/10.3390/app11062473
Analysis of the behavior of 2D monolayers and 3D spheroid human pancreatic beta cells derived from induced pluripotent stem cells in a microfluidic environment
Amal Essaouiba, Rachid Jellali, Marie Shinohara, Benedikt Scheidecker, Cecile Legallais, Yasuyuki Sakai, Eric Leclerc
Journal of Biotechnology,2021 330:25-56.
https://doi.org/10.1016/j.jbiotec.2021.02.009
Application of a Thin-Film Transistor Array for Cellular-Resolution Electrophysiology and Electrochemistry
Anne-Claire Eiler, Pierre-Marie Faure, Junichi Sugita, Satoshi Ihida, Dongchen Zhu, Yasuyuki Sakai, Katsuhito Fujiu, Kikuo Komori, Hiroshi Toshiyoshi, and Agnes Tixier-Mita
IEEE Transactions on Electron Devices,68(4), 2041-2048 (2021).
https://doi.org/10.1109/TED.2021.3050432
Enhancement of human hepatocyte functions by coculture with hiPS-derived intestinal cells in a pneumatic-pressure-driven two-organ microphysiological system
Marie Shinohara, Hiroshi Arakawa, Yuuichi Oda, Nobuaki Shiraki, Shinji Sugiura, Takumi Nishiuchi, Taku Satoh, Keita Iino, Sylvia Leo, Yusuke Kato, Karin Araya, Takumi Kawanishi, Took Nakatsuji, Manami Mitsuta, Kosuke Inamura, Tomomi Goto, Kenta Shinha, Wataru Nihei, Kikuo Komori,
Masaki Nishikawa, Shoen Kume, Yukio Kato, Toshiyuki Kanamori, Yasuyuki Sakai, Hiroshi Kimura
Sci. Rept.,11, 5437 (2021).
https://doi.org/10.1038/s41598-021-84861-y
Multi-omics analysis of hiPSCs-derived HLCs matured on-chip revealed patterns typical of liver regeneration
Danoy, Mathieu; Tauran, Yannick; Poulain; Jellali, Rachid; Bruce,
Johanna; Leduc, Marjorie; Le Gall; Gilard, Francoise; Kido, Taketomo;
Arakawa, Hiroshi; Araya, Karin; Mori, Daiki; Kusuhara, Hiroyuki; Kato,
Yukio; Plessy, Charles; Miyajima, Atsushi; Sakai, Yasuyuki; Leclerc,
Eric
Biotecnol. Booeng.,2021; 1– 17.
https://doi.org/10.1002/bit.27667
High density culture of pancreatic islet-like 3D tissue organized in oxygen-permeable porous scaffolds with external oxygen supply
Fumiya Tokito, Marie Shinohara, Masashi Maruyama, Kosuke Inamura, Masaki
Nishikawa, Yasuyuki Sakai
J. Biosci. Bioeng.,131(5), 543-548 (2021).
https://doi.org/10.1016/j.jbiosc.2020.12.009
Sessile liquid marbles with embedded hydrogels as 1 bioreactors for three-dimensional cell culture
Raja Vadivelu, Navid Kashaninejad, Mohammad Reza Nikmaneshi, Rubina Rahaman Khadim,
Seyedeh Sarah Salehi, Naveen Chintala Ramulu, Yasuyuki Sakai, Masaki
Nishikawa, Bahar Firoozabadi, Nam-Trung Nguyen
Advanced Biology, 5: 2170022.
https://doi.org/10.1002/adbi.202170022
3D perfusion culture of mouse insulinoma in macro-porous scaffolds enhanced insulin production response
Changsorn, Karn; Pang, Yuan; Matsumoto, Hiroaki; Wuthrich, Pierre; Sun, Wei;
Sakai, Yasuyuki,
The International Journal of Artificial Organs.
https://doi.org/10.1177/0391398820985515
Bioelectrochemical detection of histamine release from basophilic leukemia cell line based on histamine dehydrogenase-modified cup-stacked carbon nanofibers
Kikuo Komori, Yuto Komatsu, Masaharu Nakane, Yasuyuki Sakai
Bioelectrochemistry, 138 (2021) 107719.
https://doi.org/10.1016/j.bioelechem.2020.107719
Protection of human induced pluripotent stem cells against shear stress in suspension culture by Bingham plastic fluid
Ikki Horiguchi, Fuad Gandhi Torizal, Hotaka Nagate, Haruka Inose, Kousuke Inamura, Osamu Hirata, Hisato Hayashi, Masato Horikawa, Yasuyuki Sakai
Biotechnology Progress, 37, 2, e3100.
https://doi.org/10.1002/btpr.3100
2020
Microfluidic System with Extended‐Gate‐Type Organic Transistor for Real‐Time Glucose Monitoring
Pierre Didier, Nicolas Lobato‐Dauzier, Nicolas Clément, Anthony J. Genot, Yui Sasaki, Éric Leclerc, Tsukuru Minamiki, Yasuyuki Sakai, Teruo Fujii, Tsuyoshi Minami
ChemElectroChem, 7, 1332 –1336 (2020); https://doi.org/10.1002/celc.201902013
Development of a pancreas-liver organ-on-chip coculture model for organ-to-organ interaction studies
Amal Essaouiba, Teru Okitsu, Rie Kinoshita, Rachid Jellali, Marie Shinohara, Mathieu Danoy, CécileLegallais, Yasuyuki Sakai, Eric Leclerc
Biochemical Engineering Journal, 164, 107783 (2020); https://doi.org/10.1016/j.bej.2020.107783
Microwell-based pancreas-on-chip model enhances genes expression and functionality of rat islets of Langerhans
Amal Essaouiba, Teru Okitsu, Rachid Jellali, Marie Shinohara, Mathieu Danoy, Yannick Taurane, Cécile Legallais, Yasuyuki Sakai, Eric Leclerc
Molecular and Cellular Endocrinology, 514, 110892 (2020); https://doi.org/10.1016/j.mce.2020.110892
Modular assembly–based approach of loosely packing co-cultured hepatic tissue elements with endothelialization for liver tissue engineering
Jianyu He, Yuan Pang, Huayu Yang, Kevin Montagne, Marie Shinohara, Yilei Mao, Wei Sun, Yasuyuki Sakai
Annals of Translational Medicine, 21, 1400 (2020);
https://doi.org/10.21037/atm-20-1598Kinetic analysis of sequential metabolism of triazolam and its extrapolation to humans using an entero-hepatic two-organ microphysiological system
Hiroshi Arakawa, Shinji Sugiura, Takumi Kawanishi, Kazumi Shin, Hiroko Toyoda, Taku Satoh, Yasuyuki Sakai, Toshiyuki Kanamori, Yukio Kato
Lab Chip, 2020,20, 537-547,
https://doi.org/10.1039/C9LC00884E
Analysis of hiPSCs differentiation toward hepatocyte-like cells upon extended exposition to oncostatin
Mathieu Danoy, Yannick Tauran, Stephane Poulain, Hiroshi Arakawa, Daiki Mori, Karin Araya, Sachi Kato, Taketomo Kido, Hiroyuki Kusuhara, Yukio Kato, Atsushi Miyajima, Charles Plessy, Yasuyuki Sakai, Eric Leclerc
Differentiation, 114, 36-48 (2020);
https://doi.org/10.1016/j.diff.2020.05.006
Design, fabrication, and evaluation of polyglycolic acid modules with canals as tissue elements in cellular assembly technology
Jingyuan Ji, Yuan Pang *, Stephanie Sutoko, Yohei Horimoto, Wei Sun, Toshiki Niino, Yasuyuki Sakai
Applied Sciences, 10(11), 3748 (2020);
https://doi.org/10.3390/app10113748
Induction of in vitro Metabolic Zonation in Primary Hepatocytes Requires Both Near-Physiological Oxygen Concentration and Flux
Benedikt Scheidecker, Marie Shinohara, Masahiro Sugimoto, Mathieu Danoy, Masaki Nishikawa, and Yasuyuki Sakai
Frontiers in Bioengineering and Biotechnology, section Tissue Engineering and Regenerative Medicine, 8, 524 (2020);
https://doi.org/10.3389/fbioe.2020.00524
A tubular bile duct structure mimicking bile duct morphogenesis for prospective in vitro liver metabolite recovery
Astia Rizki-Safitri, Marie Shinohara, Minoru Tanaka, and Yasuyuki Sakai
Journal of Biological Engineering, 2020 Mar 19;14:11
https://doi.org/10.1186/s13036-020-0230-z
Characterization of liver zonation-like transcriptomic patterns in HLCs derived from hiPSCs in a microfluidic biochip environment
Mathieu Danoy, Stephane Poulain, Myriam Lereau-Bernier, Sachi Kato, Benedikt Scheidecker, Taketomo Kido, Atsushi Miyajima, Yasuyuki Sakai, Charles Plessy, Eric Leclerc
Biotechnology Progress, e3013 (2020)
https://doi.org/10.1002/btpr.3013
Integration of metabolomic and transcriptomic profiles of hiPSCs-derived hepatocytes in a microfluidic environment
Danoy, M., Poulain, S., Jelalli, R., Gilard, F., Kato, S., Plessy, C., Kido, T., Miyajima, A., Sakai, Y., & Leclerc, E.
Biochemical Engineering Journal, 155, 107490,(2020)
https://doi.org/10.1016/j.bej.2020.107490
Transcriptome profiling of hiPSCs-derived LSECs with nanoCAGE
Danoy, M., Poulain, S., Koui, Y., Tauran, Y., Kido, T., Miyajima, A., Sakai, Y., Plessy, C., & Leclerc, E
Molecular Omics, 2020, Advance Article, (2020)
https://doi.org/10.1039/C9MO00135B
Organization of liver organoids using Raschig ring-like micro-scaffolds and triple co-culture: Toward modular assembly-based scalable liver tissue engineering
Pang, Y., Sutoko, S., Wang, Z., Horimoto, Y., Montagne, K., Horiguchi, I., Shinohara, M., Danoy, M., Niino, T., Sakai, Y
Medical Engineering & Physics, 76, 69-78 (2020)
https://doi.org/10.1016/j.medengphy.2019.10.010
Integration of metabolomic and transcriptomic profiling to compare two protocols of differentiation of human induced pluripotent stem cells into hepatocytes
Jellali, R., Poulain, S., Bernier, M. L., Gilard, F., Tauran, Y., Kato, S., Danoy, M., Segard, B., D., Kido, T., Miyajima, A., Plessy, C., Sakai, Y., Leclerc, E.
Process Biochemistry, 88, 138-147, (2020)
https://doi.org/10.1016/j.procbio.2019.09.034
Metabolomic profiling during the differentiation of human induced pluripotent stem cells into hepatocyte-like cells
Jellali, R., Bernier, M. L., Tauran, Y., Gilard, F., Danoy, M., Kido, T., Miyajima, A., Sakai, Y., Leclerc, E..
Differentiation. 112, 17-26 (2020)
https://doi.org/10.1016/j.diff.2019.10.006
2019
Cytoplasmic fusion between an enlarged embryonic stem cell and a somatic cell using a microtunnel device
Seong Min Kim; Ken-Ichi Wada; Masashi Ueki; Kazuo Hosokawa; Yasuyuki Sakai; Mizuo Maeda; Yoshihiro Ito
Biochemical and Biophysical Research Communications (BBRC), 520, 257-262 (2019)
Improved isolation of mesenchymal stem cells based on interactions between N-acetylglucosamine-bearing polymers and cell-surface vimentin
Hirohiko Ise, Kumiko Matsunaga, Marie Shinohara and Yasuyuki Sakai
Stem Cells International, vol. 2019, 13, (2019)
Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device.
Matsumoto S, Safitri AR, Danoy M, Maekawa T, Kinoshita H, Shinohara M, Sakai Y, Fujii T, Leclerc E.
Biotechnol Prog. 2019 May 26:e2854.
Micropatterned nanolayers immobilized with nerve growth factor for neurite formation of PC12 cells,
Seong Min Kim, Masashi Ueki, Xueli Ren, Jun Akimoto, Yasuyuki Sakai, and Yoshihiro Ito
International Journal of Nanomedicine, 14, 2019, 7683-7694 (2019)
Metabolome Analysis Reveals Dermal Histamine Accumulation in Murine Dermatitis Provoked by Genetic Deletion of P-Glycoprotein and Breast Cancer Resistance Protein.
Hashimoto N, Nakamichi N, Nanmo H, Kimura KI, Masuo Y, Sakai Y, Schinkel AH, Sato S6, Soga T, Kato Y.
Pharm Res. 2019 Sep 11;36(11):158. (2019)
Biodegradable and hollowed micro-scaffolds for improved modular assembly-based tissue engineering: design, 3D fabrication, and feasibility in randomly packed perfusion culture,
Stephanie Sutoko, Yuan Pang, Yohei Horimoto, Kevin Montagne, Kikuo Komori, Kiyoshi Takano, Ryo Shirakashi, Masahiro Anzai, Toshiki Niino, Yasuyuki Sakai,
Biochem. Eng. J., 149, 107239 (2019)
Improvement of oxygen supply to the multicellular spheroids using a gas-permeable plate and embedded hydrogel beads,
Hirotaka Mihara , Mai Kugawa , Kanae Sayo , Fumiya Tao , Marie Shinohara , Masaki Nishikawa , Yasuyuki Sakai , Takeshi Akama , Nobuhiko Kojima,
Cells, 2019, 8, 525 (2019)
Switching of cell proliferation/differentiation in thiol-maleimide clickable microcapsules triggered by in situ conjugation of biomimetic peptides,
Oki, Yuichiro; Kirita, Katsuhisa; Ohta, Seiichi; Ohba, Shinsuke; Horiguchi, Ikki; Sakai, Yasuyuki; Ito, Taichi,
Biomacromolecules, 2019, 20, 6, 2350-2359 (2019)
Size-Dependent Hepatic Differentiation of Human Induced Pluripotent Stem Cells Spheroid in Suspension Culture,
Fuad Gandhi-Torizal, Keiichi Kimura, Ikki Horiguchi, Yasuyuki Sakai,
Regen. Ther, 12, 66-73 (2019)
A novel tool for suspension culture of human induced pluripotent stem cells: Lysophospholipids as a cell aggregation regulator
M. Ibuki, I. Horiguchi, Y. Sakai
Regen. Ther, 12, 74-82 (2019)
Endodermal differentiation of human induced pluripotent stem cells using simple dialysis culture system in suspension culture
M. Shinohara, H. Choi, M. Ibuki, S. G.Yabe, H. Okochi, A. Miyajimam Y. Sakai
Regen. Ther, 12, 14-19 (2019)
Size-controlled preparation of microsized perfluorocarbon emulsions as oxygen carriers via the Shirasu porous glass membrane emulsification technique
X. T. Fu, S. Ohta, M. Kamihira, Y. Sakai and T. Ito
Langmuir, 35 (11), pp 4094-4100, (2019)
Analysis of the transcription factors and their regulatory roles during a step-by-step differentiation of induced pluripotent stem cells into hepatocyte-like cells
Tauran, Y., Poulain, S., Lereau-Bernier, M., Danoy, M., Shinohara, M., Segard, B. D., Kato, S., Kido, T., Miyajima, A., Sakai, Y., Plessy, C., Leclerc, E.
Molecular omics, 15(6), 383-398 (2019)
Optimized protocol for the hepatic differentiation of iPSCs in a fluidic microenvironment
Mathieu Danoy, Myriam Lereau Bernier, Keiichi Kimura, Stephane Poulain,
Sachi Kato, Daiki Mori, Taketomo Kido, Charles Plessy, Hiroyuki Kusuhara,
Atsushi Miyajima, Yasuyuki Sakai, Eric Leclerc
Biotechnology and Bioengineering, 116(7), 1762-1776 (2019)
Profiling of derived-hepatocyte progenitors from induced pluripotent stem cells using nanoCAGE promoter analysis
Myriam Lereau Bernier, Stéphane Poulain, Yannick Tauran, Mathieu Danoy, Marie Shinohara, Keiichi Kimura, Bertrand David Segard, Sachi Kato, Taketomo Kido, Atsushi Miyajima, Yasuyuki Sakai, Charles Plessy, Éric Leclerc
Biochemical engineering journal, 142, 7-17 (2019)
Physiological Microenvironmental Conditions in Different Scalable Culture Systems for Pluripotent Stem Cell Expansion and Differentiation
Fuad G. Torizal, Ikki Horiguchi, Yasuyuki Sakai
The Open Biomedical Engineering Journal., 13, 41-54 (2019)
Enhanced hepatic differentiation of human iPS cells using gas permeable membrane
Keiichi Kimura, Ikki Horiguchi, Taketomo Kido, Atsushi Miyajima, Yasuyuki Sakai
Tissue Eng. Part A, 25, 5-6 (2019)
An orbital shaking culture of mammalian cells in O-shaped vessels for the production of uniform aggregates
Ikki Horiguchi, Ikumi Suzuki, Takashi Morimura, Yasuyuki Sakai
J. Vis. Exp., (143), e57922, doi:10.3791/57922 (2019).
2018
- Integration of an oxygen sensor into polydimethylsiloxane hepatic culture device for two-dimensional gradiate characterization,
T. Matsumoto, E. Leclerc, T. Mekawa, H. Kinoshita, M. Shinohara, K. Komori, Y. Sakai, T. Fujii,
Sens. Actuat. B Chem., 273, 1062-1069 (2018).
Development of human-derived hemoglobin/albumin microspheres as oxygen carriers using Shirasu porous glass membrane emulsification,
S. Ohta, K. Hashimoto, X. T. Fu, M. Kamihira, Y. Sakai, and T. Ito,
Journal of Bioscience and Bioengineering, 126 (4), pp 533-539, (2018).
Efficient functional cyst formation of biliary epithelial cells using microwells for potential bile duct organisation in vitro
A. Rizki-Safitri, M. Shinohara, Y. Miura, M Danoy, M Tanaka, A Miyajima, Y. Sakai
Scientific Reports, 8(1), 11086 (2018).
Effects of glucose, lactate and basic FGF as limiting factors on the expansion of human induced pluripotent stem cells
Ikki Horiguchi, Yusuke Urabe, Keiichi Kimura, Yasuyuki Sakai
J. Biosci. Bioeng., 125(1), 111-115 (2018).
2017
- Enhanced self-organization of size-controlled hepatocyte aggregates on oxygen permeable honeycomb microwell sheets
Marie Shinohara, Kikuo Komori, Teruo Fujii, Yasuyuki Sakai
Biomedical Physics & Engineering Express, 3(4), 045017 (2017). - Alteration of pancreatic carcinoma and promyeloblastic cell adhision in liver microvasculature by coculture of hepatocytes, hepatic stellate cells and endothelial cells in a physiologically-relevant model
Mathieu Danoy, Marie Shinohara, Astia Rizki-Safitri, Dominique Collard, Vincent Senez, Yasuyuki Sakai
Integrative Biology, 9(4), 350-361 (2017). - Comparison of the transcriptomic profile of hepatic human induced pluripotent stem like cells cultured in Petri and in a 3D microscale dynamic environment
Eric Leclerc, Keiichi Kimura, Marie Shinohara, Mathieu Danoy, Morgane Le Galld, Taketomo Kido, Atsushi Miyajima, Teruo Fujii, Yasuyuki Sakai
Genomics, 109(1), 16-26 (2017). 2016
- Novel integrative methodology for engineering large liver tissue equivalents based on three-dimensional scaffold fabrication and cellular aggregate assembly
Y. Pang, Y. Horimoto, S. Sutoko, K. Montagne, M. Shinohara, M. Danoy, K. Komori, M. Anzai, T. Niino, Y. Sakai
Biofabrication, 8(3), 035016 (2016) - Direct Electron Transfer Kinetics of Peroxidase at Edge Plane Sites of Cup-Stacked Carbon Nanofibers and their Comparison with Single-Walled Carbon Nanotubes
K. Komori, T. Tatsuma, Y. Sakai
Langmuir, 32, 9163-9170 (2016). - Serum replacement with albumin-associated lipids prevents excess aggregation and enhances growth of induced pluripotent stem cells in suspension culture
Ikki Horiguchi and Yasuyuki Sakai
Biotechnology progress, 32(4), 1009-16 (2016). - Highly Efficient and Gentle Trapping of Single Cells in Large Microfluidic Arrays for Time-lapse Experiments
Filiz Yesilkoy, Ryohei Ueno, Benoit Xavier Emmanuel Desbiolles, Marco Grisi, Yasuyuki Sakai, Beomjoon Kim, Juergen Brugger
Biomicrofluidics, 10(1), 014120 (2016). - Oxygenated Cup-Stacked Carbon Nanofibers/TiO2 Composite Films with Enhanced Photocatalytic Currents
K. Komori, K. Yamura, A. Kogo, Y. Takahashi, T. Tatsuma, A. Sakoda, Y. Sakai
Bull. Chem. Soc. Japan., 89, 603-607 (2016).
Selected paper of issue - Production of cisplatin-incorporating hyaluronan nanogels via chelating ligand-metal coordination.
S. Ohta, S. Hiramoto, Y. Amano, M. Sato, Y. Suzuki, M. Shinohara, S. Emoto, H. Yamaguchi, H. Ishigami, Y. Sakai, J. Kitayama, T. Ito, Bioconjug. Chem., 27(3), 504-8 (2016). - Three-dimensional graphene: biocompatible and biodegradable scaffold with enhanced oxygenation for HepG2 liver cells culture
Manuela Loeblein, Guillaume Perry, Siu Hon Tsang, Wenjin Xiao, Dominique Collard, Philippe Coquet, Yasuyuki Sakai, Edwin Hang Tong Teo*
Advanced Healthcare Materials, 5(10), 1177-91 (2016). 2015
- New physiologically-relevant liver tissue model based on hierarchically cocultured primary rat hepatocytes with liver endothelial cells.
Xiao W., Perry G., Komori K., Sakai Y., Inteqr. Biol., 11, 1412-22, (2015) - Establishment of a new liver tissue model by hierarchically coculturing primary rat hepatocytes with liver sinusoidal endothelial cells on a gas-permeable membrane
Wenjin XIAO, Guillaume PERRY, Kikuo KOMORI, Yasuyuki SAKAI
Integrative Biology, 7(11), 1412-22 (2015). - Alginate encapsulation of pluripotent stem cells using a co-axial nozzle
I. Horiguchi and Y. Sakai
J. Visualized. Exp., 101, e52835 (2015). - Size-dependent interaction of cells and hemoglobin-albumin based oxygen carriers prepared using the SPG membrane emulsification technique.
Lai YT, Ohta S, Akamatsu K, Nakao SI, Sakai Y, Ito T. Biotechnol. Prog., 31(6), 1676-84 (2015). - Preparation of uniform-sized hemoglobin-albumin microspheres as oxygen carriers by Shirasu porous glass membrane emulsification technique
Lai YT, Sato M, Ohta S, Akamatsu K, Nakao SI, Sakai Y, Ito T.
Colloids. Surf. B Biointerfaces., 127, 1-7 (2015). 2014
- Wenjin XIAO, Hitoshi MATSUI, Marie SHINOHARA, Kikuo KOMORI, Tomoharu OSADA, Yasuyuki SAKAI
The importance of physiological oxygen concentrations in the sandwich cultures of rat hepatocytes on gas-permeable membranes
Biotechnology Progress, 2014 Nov-Dec;30(6):1401-10. - Ikki Horiguchi, Mohammad M. Chowdhury, Yoji Tabata, Yasuyuki Sakai
Proliferation, morphology and pluripotency of mouse induced pluripotent stem cells (iPSCs) in three different types of alginate beads for mass production
Biotechnol. Prog.,2014 Jul-Aug;30(4):896-904. - Mohammad Mahfuz Chowdhury, Mathieu Danoy, Farhana Rahman, Marie Shinohara, Shohei Kaneda, K. Shiba, Naoya Fujita, Teruo Fujii and Yasuyuki Sakai
Adhesion of pancreatic cancer cells in a liver-microvasculature mimicking co-culture correlates with their propensity to form liver-specific metastasis in vivo
BioMed Research International, Special issue on Hepatocellular Carcinoma: Carcinogenesis, Establishment, Progression, and Therapies
, in press. - Kojima, N., Takeuchi, S. and Sakai, Y.
Fabrication of microchannel networks in multicellular spheroids
Sensors and Actuators B 198 (2014) 249–254. - S. Ko, T. Tatsuma, A. Sakoda, Y. Sakai, and K. Komori, "Electrochemical
Properties of Oxygenated Cup-Stacked Carbon Nanofiber-Modified Electrodes",
Phys. Chem. Chem. Phys.,2014 Jun 28;16(24):12209-13 - N. Kojima, S. Takeuchi Y. Sakai
"Engineering of Pseudoislets: Effect on Insulin Secretion Activity by
Cell Number, Cell Population, and Microchannel Networks"
Transplant. Proc.,2014 May;46(4):1161-5. doi: 10.1016 - H. Nakayama, H. Kimura, T. Fujii, Y. Sakai
"Image-based evaluations of distribution and cytotoxicity of Irinotecan
(CPT-11) in a multi-compartment micro-cell coculture device"
J. Biosci. Bioeng.,2014 Jun;117(6):756-62. - Y. Tabata, I. Horiguchi, M. P. Lutolf, Y. Sakai
Development of bioactive hydrogel capsules for the 3D expansion of pluripotent stem cells in bioreactors
Biomat. Sci.,2,176-183 (2014). - M. Shinohara, H. Kimura, K. Montagne, K. Komori, T. Fujii, Y. Sakai
"Combination of microwell structures and direct oxygenation enables efficient
and size-regulated aggregate formation of an insulin-secreting pancreatic beta-cell line"
Biotechnol. Prog.,30, 178-187, (2014). 2013
- T. Katsuda, N. Kojima, T. Ochiya and Y. Sakai
Biliary epithelial cells play an essential role in the reconstruction of hepatic tissue with a functional bile ductular network
Tissue Eng. A, 19(21-22), 2402-2411 (2013). - N. Kojima, Y. Sakai
Control of liver tissue reconstitution in mesenteric leaves: the effect of preculture on mouse hepatic progenitor cells prior to transplantation
J. Robotics Mechatronics, 25(4), 698-704 (2013). - K. Iwasawa, G. Tanaka, T. Aoyama, M. M. Chowdhury, K.Komori, T. Tanaka-Kagawa, H. Jinno, Y. Sakai
Prediction of phthalate permeation through pulmonary alveoli using a cultured A549 cell-based in vitro alveolus model and a numerical simulation
, AATEXm Vol. 18 (2013) No. 1 p. 19-31 - YT. Lai, S. Ohta, K. Akamatsu, S. Nakao Y. Sakai, T. Ito
Preparation of uniform-sized poly[methacryloxypropyl tris(trimethylsiloxy)silane] microspheres via Shirasu porous glass membrane emulsification technique
J. Chem. Eng. Jpn.,Vol. 46 (2013) No. 11 p. 777-784 - K. Komori, M. Udagawa, M. Shinohara, K. Montagne, T. Tsuru, and Y. Sakai
“Formation and Harvesting of Thick Pancreatic β-Cell Sheets on a Highly
O2-Permeable Plate Modified with Poly(N-isopropylacrylamide)”, Biomaterials
Science, 1, 510-518 (2013). - T. Katsuda, R.Tsuchiya, N. Kosaka, Y.Yoshioka, K. Takagaki, K.Oki, F.Takeshita, Y. Sakai, M.Kuroda and T Ochiya
Human adipose tissue-derived mesenchymal stem cells secrete functional neprilysin-bound exosomes
Scientific Reports (2013), DOI: 10.1038/srep01197 - T. Katsuda, T.Teratani, M. M. Chowdhury, T. Ochiya and Y.Sakai
Hypoxia efficiently induces differentiation of mouse embryonic stem cells into endodermal and hepatic progenitor cells
Biochemical Engineering Journal 74 95-101 (2013). - M. M. Chowdhury, T. Fujii and Y. Sakai
Importance of a diffusion-dominant small volume to activate cell-secreted soluble factor signaling in embryonic stem cell culture in microbioreactors: A mathematical model based study
Journal of Bioscience and Bioengineering (2013),DOI: 10.1016/j.jbiosc.2013.01.008. - [[ 2012 ] ]
- Y. Pang, K. Montagne, M. Shinohara, K. Komori, and Y. Sakai
Liver tissue engineering based on aggregate assembly: efficient formation of endothelialized rat hepatocyte aggregates and their immobilization with biodegradable fibers
Biofabrication, 4 (2012) 045004. - J. Kawada, H. Kimura, H. Akutsu, Y. Sakaiab and T. Fujii
Spatiotemporally controlled delivery of soluble factors for stem cell differentiation
Lab Chip, 12 (21), 4508 - 4515 (2012). - S. Ko, Y. Takahashi, A. Sakoda, Y. Sakai, and K. Komori*
Direct Synthesis of Cup-Stacked Carbon Nanofiber Microspheres by the Catalytic Pyrolysis of Poly(ethylene glycol)
Langmuir, 28 (23), 8760–8766 (2012). - M. M. Chowdhury, H.Kimura, T. Fujii, and Y. Sakai,
"Induction of alternative fate other than default neuronal fate of embryonic stem cells in a membrane-based two-chambered microbioreactor
by cell-secreted BMP4"
Biomicrofluidics, 6, 014117 (2012). - K. Komori, S. Fujii, K. Montagne, H. Nakamura, H. Kimura, K. Otake, T.Fujii, and Y. Sakai,
Development of the Well of the Well System-Based Embryo Culture Plate with an Oxygen Sensing Photoluminescent Probe
Sens. Actuators B, 162, 278-283 (2012). - S. Ko, Y. Takahashi, H. Fujita, T. Tatsuma, A. Sakoda, and K. Komori
Peroxidase-Modified Cup-Stacked Carbon Nanofiber Networks for Electrochemical Biosensing with Adjustable Dynamic Range
RSC Advances, 2, 1444-1449 (2012). - F. Evenou, M. Hamon, T. Fujii, S. Takeuchi, and Y. Sakai
Gas-permeable Membranes and Co-culture with Fibroblasts Enable High-density Hepatocyte Culture as Multilayered Liver Tissues
Biotechnol. Prog. 27, 1146-1153 (2012). - N. Kojima, S. Takeuchi, and Y. Sakai
Rapid aggregation of heterogeneous cells and multiple-sized microspheresin methylcellulose medium
Biomaterials, 33(18), 4508-4514 (2012). - M. Hamon, S. Hanada, T. Fujii, and Y. Sakai
Direct Oxygen Supply with Polydimethylsiloxane (PDMS) Membranes Induces a Spontaneous Organization of Thick Heterogeneous Liver Tissues from Rat Fetal Liver Cells in Vitro
Cell Transplantation, 21(2-3), 401-410 (2012). - H. Matsui, S. Takeuchi, T. Osada, T. Fujii, and Y. Sakai
Enhanced bile canaliculi formation enabling direct recovery of biliary metabolites of hepatocytes in 3D collagen gel microcavities
Lab on-a-Chip, 33(18), 4508-4514 (2012). 2011
- S. Ostrovidov, Y. Sakai, and T. Fujii
Integration of a pump and an electrical sensor into a membrane-based PDMS microbioreactor for cell culture and drug testing
Biomed. Microdev., 13(5), 847-864 (2011). - K. Montagne, H. Huang, K. Ohara, K. Matsumoto, A. Mizuno, K. Ohta, and Y. Sakai
Use of liposome encapsulated hemoglobin as an oxygen carrier for fetal and adult rat liver cell culture
J. Biosci. Bioeng, 112(5), 485-490 (2011). - T Niino, D Hamajima, K Montagne, S Oizumi, H Naruke, H Huang, Y Sakai, HKinoshita, and T Fujii
Laser sintering fabrication of three-dimensional tissue engineering scaffolds with a flow channel network
Biofabrication, 3, 034104 (2011). - T. Kurakazu, K. Kuribayashi-Shigetomi, Y. T. Matsunaga, H. Kimura, T. Fujii, Y.Sakai, and S. Takeuchi
Selective Retrieval Method Using Electrolytically Generated Bubbles for Cell Array Applications
Sensors & Actuators: B. Chemical, 159(1), 229-233 (2011). - M. Hamon, K. Montagne, N. Kojima, T. Ozawa, R. Ishii, S. Yamaguchi, T. Nagamune, T. Ushida, and Y. Sakai,
Avidin-biotin-based approach to forming heterotypic cell clusters and cell sheets on a gas-permeable membrane
Biofabrication, 3(3), 034111 (2011). - D. A. Ouattara, S.-H. Choi, Y. Sakai, A. R. R. Pérya, and C. Brochot
Kinetic modelling of in vitro cell-based assays to characterize non-specific bindings and ADME processes in a static and a perfused fluidic system
Toxicol. Lett., 205(3), 310-319 (2011). - N. Kojima, S. Takeuchi, and Y. Sakai
Establishment of self-organization system in rapidly formed multicellular heterospheroids
Biomaterials, 32(26), 6059–6067 (2011). - Ginet P, Montagne K, Akiyama S, Rajabpour A, Taniguchi A, Fujii T, Sakai Y, Kim B, Fourmy D, Volz S.
Towards single cell heat shock response by accurate control on thermal confinement with an on-chip microwire electrode
Lab on-a-Chip, 11(8):1513-20 (2011). - Yosuke Nakao, Hiroshi Kimura, Yasuyuki Sakai, and Teruo Fujii
Bile canaliculi formation by aligning rat primary hepatocyte in a microfluidic device
Biomicrofluidics, 5, 022212 (2011). - K.Montagne, R.Plasson, Y.Sakai, T.Fujii, and Y.Rondelez
Programming an in vitro DNA oscillator using a molecular networking strategy
Mol Syst Biol., 7, (Feb 1;7:466 2011). - N. Yamamoto, K. Komori, K. Montagne, H. Matsui, H. Nakayama, S. Takeuchi, and Y. Sakai
Cytotoxicity evaluation of reactive metabolites using rat liver homogenate microsome-encapsulated alginate gel microbeads
J. Biosci.Bioeng., 111(4), 454-458 (2011). - F. Evenou, S. Couderc, B. Kim, T. Fujii, and Y. Sakai
Microfibrillated Cellulose Sheets Coating Oxygen-Permeable PDMS Membranes Induce Rat Hepatocytes 3D Aggregation into Stably-Attached 3D Hemispheroids
J. Biomater. Sci. Polym. Ed., 22, 1509-1522 (2011). 2010
- M. M. Chowdhury, T. Katsuda, K. Montagne, H. Kimura, N. Kojima, H. Akutsu, T. Ochiya, T. Fujii, and Y. Sakai
Enhanced Effects of Secreted Soluble Factor Preserve Better Pluripotent State of Embryonic Stem Cell Culture in a Membrane-based Compartmentalized Micro-bioreactor
Biomed. Microdev., 12, 1097-1105 (2010). - T. Katsuda, T. Teratani, T. Ochiya, and Y. Sakai
Transplantation of a Fetal Liver Cell-loaded Hyaluronic Acid Sponge onto the Mesentery Recovers a Wilson's Disease Model Rat
J. Biochem., 148 (3), 281-288 (2010). - H. Kimura, H. Takeyama, K. Komori, T. Yamamoto, Y. Sakai, and T. Fujii
Microfluidic Device with Integrated Glucose Sensor for Cell-based Assay in Toxicology
J. Robotics Mech., 22, 594-600 (2010). - N. Kojima, K. Miura, T. Matsuo, H. Nakayama, K. Komori, S. Takeuchi, and Y. Sakai
Rapid and Direct Cell-to-Cell Attachment Using Avidin-Biotin Binding System: Large Aggregate Formation in Suspension Culture and Small Tissue Element Formation Having a Precise Microstructure Using Optical Tweezers
J. Robotics Mech., 22, 619-622 (2010). - Q. Rui, K. Komori, Y. Tian, H. Liu, Y. Luo, and Y. Sakai
Electrochemical Biosensor for Detection of H2O2 from Living Cancer Cells Based on ZnO Nanosheets
Anal. Chim. Acta, 670, 57-62 (2010). - N. Pereira-Rodrigues, P. E. Poleni, D. Guimard, Y. Arakawa, Y. Sakai, and T. Fujii
Modulation of Hepatocarcinoma Cell Morphology and Activity by Parylene-C Coating on PDMS
PloS One, 5, e9667 (2010). - F. Evenou, T. Fujii, and Y. Sakai
Spontaneous Formation of Stasbly-attached and 3D-organized Hepatocyte Aggregates on Oxygen-permeable Polydimethylsiloxiane Membrane Having 3D Microstrucctures
Biomedical Microdevices, 12, 465-475 (2010). - F. Evenou, T. Fujii, and Y. Sakai
Spontaneous Formation of Highly Functional Three-dimensional Multilayer from Human Hepatoma Hep G2 Cells Cultured on an Oxygen-permeable Polydimethylsiloxane Membrane
Tissue Engineering Part C: Methods, 16, 311-318 (2010). - H. Kimura, H. Nakamura, T. Akai, T. Yamamoto, H. Hattori, Y. Sakai, and T. Fujii
Study of On-chip Individual Embryo Coculture with Microporous Membrane Supported Endometrial Cells
IEEE Trans. NanoBiosci., 8(4), 318-324 (2010). 2009
- K. Montagne, K. Komori, F. Yang, T. Tatsuma, T. Fujii, and Y. Sakai
A Micropatterned Cell Array with an Integrated Oxygen-sensitive Fluorescent Membrane
Photochemistry & Photobiological Sciences, 8, 1529-1533 (2009). - K. Komori, J. Nada, M. Nishikawa, H. Notsu, T. Tatsuma, and Y. Sakai
Simultaneous Evaluation of Toxicities Using a Mammalian Cell Array Chip Prepared by Photocatalytic Lithography
Analytica Chimica Acta, 653, 222-227 (2009). - K. Komori, S. Miyajima, T. Tsuru, T. Fujii, S. Mohri, Y. Ono, and Y. Sakai
A Rapid and Simple Evaluation System for Gas Toxicity Using Luminous Bacteria Entrapped by a Polyion Complex Membrane
Chemosphere, 77, 1106-1112 (2009). - K. Komori, S. Takamiya, N. Kojima, O. Kuwazuru, N. Yoshikawa, K. Furukawa, K. Miyamoto, and Y. Sakai
Initial Characterization of a Fibroblast-loaded Porous Elastin Film Reconstituted by a Novel Cross-lonker, Dode-DSP
Chemistry Letters, 38, 878-879 (2009). - C. Provin, K. Takano, T. Yoshida, Y. Sakai, T. Fujii, and R. Shirakashi
Low O2 metabolism of Hep G2 cells cultured at high cell density in a 3D microstructured scaffolds
Biomedical Microdevices, 11(2), 485-494 (2009). 2008
- K. Ishii, Y. Kikukawa, M. Shiine, N. Kobayashi, T. Tsuru, Y. Sakai, and A. Sakoda
Synthesis and Photophysical Properties of Silica-Gel-Supported Photofunctional (Phthalocyaninato)silicon Complexes
European Journal of Inorganic Chemistry, 2975-2981 (2008). - V. Senez, E. Lennon, S. Ostrovidov, T. Yamamoto, H. Fujita, Y. Sakai, and T. Fujii
Integrated 3-D Silicon Electrodes for Electrochemical Sensing in Microfluidic Environments: Application to Single-Cell Characterization
IEEE Sensors Journal, 8, 548-557 (2008). - R. Shoji, H. Nakayama, Y. Sakai, S. Mohri, and M. Yamada
Evaluation of the ecotoxicity of solid wastes using rapid leaching test and bioassays
J. Envron. Sci. Health Part A, 43, 1048-1053 (2008). - R. Shoji, S. Mohri, Y. Sakai, and M. Yamada
Ecotoxicity Assessment of Sludge and Leaching Test Elutes of Sludge
J. Envron. Sci. Health Part A, 43, 1042-1047 (2008). - K. Komori, K. Murai, S. Miyajima, T. Fujii, S. Mohri, Y. Ono, and Y. Sakai
Development of an in vitro Batch Type-Closed Gas Exposure Device with an Alveolar Epithelial Cell Line, A549, for Toxicity Evaluations of Gaseous Compounds
Analytical Sciences, 24, 957-962 (2008) [Hot article and on the cover of the issue]. - H. Kimura, H. Sakai, T. Yamamoto, Y. Sakai, and T. Fujii
An Integrated Microfluidic System for Long-term Perfusion Culture and On-line Monitoring of Intestional Tissue Models
Lab on a Chip, 8, 741-746 (2008). - C. Provin, K. Takano, Y. Sakai, T. Fujii, and R. Shirakashi
A Method for the Design of 3D Scaffolds for High-density Cell Attachment and Determination of Optimun Perfusion culture conditions
Journal of Biomechanics, 41, 1436-1449 (2008). - N. P. Rodigues, Y. Sakai, and T. Fujii
Cell-Based Microfluidic Biochip for the Electrochemical Real-Time Monitoring of Glucose and Oxygen
Sensors & Actuators: B, Chemical, 132, 608-613 (2008). - S. Hanada, N. Kojima, and Y. Sakai
Soluble Factor-Dependent in vitro Growth and Maturation of Rat Fetal Liver Cells in a Three-Dimensional Culture System
Tissue Engineeing, 14, 149-160 (2008). - M. Nishikawa, T. Yamamoto, N. Kojima, K. Komori, T. Fujii, and Y. Sakai
Stable Immobilization of Rat Hepatocytes as Hemispheroids onto Collagen-conjugated Poly-dimethylsiloxane (PDMS) Surfaces: Importance of Direct Oxygenation Through PDMS for Both Formation and Function
Biotechnol. Bioeng., 99, 1472-2481 (2008). - M. Nishikawa, N. Kojima, K. Komori, T. Yamamoto, T. Fujii, and Y. Sakai
Enhanced Maintenance and Functions of Rat Hepatocytes Induced by Combination of On-Site Oxygenation and Coculture with Fibroblasts
Journal of Biotechnology, 133, 253-260 (2008).
2000 - 2007
H. Naruto, H. Huang, M. Nishikawa, N. Kojima, A. Mizuno, K. Ohta, and Y. Sakai
Feasibility of Direct Oxygenation of Primary-Cultured Rat Hepatocytes Using Polyethylene Glycol Decorated Liposome-Encapsulated Hemoglobin (LEH)
Journal of Bioscience and Bioengineering, 104, 343-346 (2007).- H. Kimura, M. Nishikawa, T. Yamamoto, Y. Sakai, and T. Fujii
Microfluidic Perfusion Culture of Human Hepatocarcinoma
Journal of Robotics and Mechatronics, 19, 550-556 (2007). - H. Nakayama, H. Kimura, K. Komori, T. Fujii, and Y. Sakai
Development of a Multi-Compartment Micro-Cell Culture Device as a Future on-Chip Human: Fabrication of a Three-Compartment Device and Immobilization of Rat Mature Adipocytes for the Evaluation of Chemical Distributions
Journal of Robotics and Mechatronics, 19, 544-549 (2007). - H. Huang, S. Oizumi, N. Kojima, T. Niino, and Y. Sakai
Avidin-Biotin Binding-Based Cell Seeding and Perfusion Culture of Liver-Derived Cells in a Porous Scaffold with a Three-Dimensional Interconnected Flow-Channel Network
Biomaterials, 28, 3815-3823 (2007). - N. Kojima, T. Matsuo, and Y. Sakai
Rapid Hepatic Cell Attachment onto Biodegradable Polymer Surfaces without Toxicity Using an Avidin-Biotin Binding System
Biomaterials, 27(28), 4904-4910 (2006). - H. Huang, S. Hanada, N. Kojima, and Y. Sakai
Enhanced Functional Maturation of Fetal Porcine Hepatocytes in Three-dimensional Poly-L-lactic Acid Scaffolds: A Culture Condition Suitable for Engineered Liver Tissues in Large-Scale Animal Studies
Cell Transplantation, 15(8-9), 799-809 (2006). - Y. Cho, T. Yamamoto, Y. Sakai, T. Fujii, and B. Kim
Development of microfluidic device for electrical/physical characterization of single cell
J. Microelectromechanical systems, 15(2), 287-295 (2006). - M. Sagehashi, T. Fukuda, T. Fujii, Y. Sakai and A. Sakoda
Elution and adsorptive concentration of Japanese cedar (Cryptomeria japonica) pollen allergen in environmental water
Water Science & Technology, 52(8), 37-43 (2005). - I. Shitanda, K. Takada, Y. Sakai, and T. Tatsuma
Amperometric Biosensing Systems Based on Motility and Gravitaxis of Flagellate Algae for Aquatic Risk Assessment
Analytical Chemistry, 77(20), 6715-6718 (2005). - Y. Sakai, K. Tomita, M. Suzuki, Y. Ono, and A. Sakoda
Development of a toxicity evaluation system for gaseous compounds using air-liquid interface culture of a human bronchial epithelial cell line, Calu-3
AATEX, 11(1), 59-67 (2005). - N. Kojima, N. Shiojiri, Y. Sakai, and A. Miyajima
Expression of neuritin during liver maturation and regeneration
FEBS Letters, 579(21), 4562-4566 (2005). - S. Okajima, T. Yamaguchi, Y. Sakai, and S. Nakao
Regulation of cell adhesion using a signal-responsive membrane substrate
Biotechnology and Bioengineering, 91(2), 237-243 (2005). - K. Naruse, H. Nagashima, Y. Sakai, N. Kokudo, and M. Makuuchi
Development and Perspectives of Perfusion Treatment for Liver Failure
Surgery Today, 35(7), 507-517 (2005). - S. Okajima, Y. Sakai, and T. Yamaguchi
Development of a regenerable cell culture system that senses and releases dead cells
Langmuir, 21(9), 4043-4049 (2005). - I. Shitanda, K. Takada, Y. Sakai, and T. Tatsuma
Compact amperometric algal biosensors for the evaluation of water toxicity
Analytica Chimica Acta, 530(2), 191-197 (2005). - K. Shimizu, O. Endo, S. Goto, A. Sakoda, Y. Ono, and Y. Sakai
Bioassay-based evaluation of toxicity of suspended particulate matter in humans: integrated uses of alveolar cells (A549) in air-liquid interface culture and hepatocarcinoma cells (Hep G2)
Biochemical Engineering Journal, 22(1), 1-9 (2004). - H. Shindoh, K. Naruse, Y. Sakai, and M. Makuuchi
Efficacy of immunoadsorbent devices for maintaining hepatic function in ex vivo xenogenic direct hemoperfusion
Int. J. Artif. Organs, 27(4), 294-302 (2004). - J. Jiang, N. Kojima, L. Guo, K. Naruse, M. Makuuchi, A. Miyajima, W. Yan, and Y. Sakai
Efficacy of Engineered Liver Tissue Based on Poly-L-lactic Acid Scaffolds and Fetal Mouse Liver Cells Cultured with Oncostatin M, Nicotinamide, and Dimethyl Sulfoxide
Tissue Engineering, 10(9/10), 1577-1586 (2004). - S. Ostrovidov, J. Jiang, Y. Sakai, and T. Fujii
Membrane-Based PDMS Microbioreactor for Perfused 3D Primary Rat Hepatocyte Cultures
Biomedical Microdevices, 6(4), 279-287 (2004). - E. Leclerc, K. S. Furukawa, F. Miyata, Y. Sakai, T. Ushida, and T. Fujii
Fabrication of microstructures in photosensitive biodegradable polymers for tissue engineering applications
Biomaterials, 25, 4683-4690 (2004) - Y. Sakai, M. Otsuka, J. Kozasa, F. Miyata, K. S. Furukawa, and A. Yamashita
Ultraviolet-irradiation-based photofabrication that simultaneously produces a macroporous structure and flow channels using a photoreactive biodegradable polymer and a gas-forming azoamide compound
Biochemical Engineering Journal, 20(2-3), 163-171 (2004). - E. Leclerc, Y. Sakai, and T. Fujii
Perfusion Culture of Fetal Human Hepatocytes in Microfluidic-Environments
Biochemical Engineering Journal, 20, 143-148 (2004). - Y. Sakai, M. Otsuka, S. Hanada, Y. Nishiyama, Y. Konishi, and A. Yamashita
A novel poly-L-lactic acid scaffold that possesses a macroporous structure and a branching/joining three-dimensional flow channel network: Its fabrication and application to perfusion culture of human hepatoma Hep G2 cells
Materials Science & Engineering, C: Biomimetic and Supramolecular Systems, 24(3), 379-386 (2004). - J. Jiang, N. Kojima, T. Kinoshita, A. Miyajima, W. Yan, and Y. Sakai
Cultivation and induction of fetal liver cells in poly-L-lactic acid scaffold
Materials Science & Engineering, C: Biomimetic and Supramolecular Systems, C24(3), 361-363 (2004). - E. Leclerc, F. Miyata, K. S. Furukawa, T. Ushida, Y. Sakai, and T. Fujii
Effect on liver cells of stepwise microstructures fabricated in a photosensitive biodegradable polymer by softlithography
Materials Science & Engineering, C: Biomimetic and Supramolecular Systems, C24(3), 349-354 (2004). - S.-H. Choi, O. Fukuda, A. Sakoda, and Y. Sakai
Enhanced cytochrome P450 capacities of Caco-2 and Hep G2 cells in new coculture system under the static and perfused conditions: evidence for possible organ-to-organ interactions against exogenous stimuli
Materials Science & Engineering, C: Biomimetic and Supramolecular Systems, C24(3), 333-339 (2004). - S.-H. Choi, M. Nishikawa, A. Sakoda, and Y. Sakai
Feasibility of a simple double-layered coculture system incorporating metabolic processes of the intestine and liver tissue: application to the analysis of benzo[a]pyrene toxicity
Toxicology in Vitro, 18(3), 393-402 (2004). - E. Leclerc, Y. Sakai, and T. Fujii
Microfluidic PDMS (Polydimethylsiloxane) Bioreactor for Large-Scale Culture of Hepatocytes
Biotechnology Progress, 20(3), 750-755 (2004). - S. Hanada, H. Kayano, J. Jiang, N. Kojima, A. Miyajima, A. Sakoda, and Y. Sakai
Enhanced in vitro maturation of subcultivated fetal human hepatocytes in three dimensional culture using poly-L-lactic acid scaffolds in the presence of oncostatin M
Int. J. Artif. Organs., 26(10), 943-951 (2003). - Y. Sakai, O. Fukuda, S.-H. Choi, and A. Sakoda
Development of a biohybrid simulator for absorption and biotransformation processes in humans based on in vitro models of the small intestine and the liver tissues
J. Artif. Organs, 6,273-281 (2003). - K Naruse, Y.Sakai, L. Guo, T. Natori, J. Shindo, Y. Karasawa, Y. Iida, K. Kojima, K. Michishita, and M. Makuuchi
Development of a new extracorporeal whole-liver perfusion system
J. Artif. Organs, 6, 211-217 (2003). - K. Naruse, Y.Sakai, T. Natori, L. Guo, J. Shindo, Y. Iida, K. Michishita, Y. Karasawa, K. Kojima, and M. Makuuchi
Xenogeneic direct hemoperfusion using whole swine liver for liver failure in dogs
J. Surg. Res., 111, 229-235 (2003). - K. S. Furukawa, H. Suenga, K. Toita, T. Ushida, Y. Sakai, and T. Tateish
Rapid and large scale formation of chondrocyte aggregates by rotational culture
Cell Transplant.,12, 475-479 (2003). - E. Leclerc, Y. Sakai, and T. Fujii
Cell culture in 3-dimensional microfluidic structure of PDMS (polydimethylsiloxane)
Biomedical Microdevices, 5(2), 109-114 (2003). - K. Naruse, Y. Sakai, L. Guo, J. Shindoh, J.-C. Son, and M. Makuuchi
Development of a xenogeneic direct hemoperfusion method in a bioartificial liver system
J. Artif. Organs., 5, 257-264 (2002). - B-S. Kim, H. Fujita, Y. Sakai, A. Sakoda, and M. Suzuki
Catalitic ozonation of an organophophorous pesticide using microporous silicate and its effect on total toxicity reduction
Water Sci. Technol., 46(4-5), 35-41 (2002). - K. Furukawa, T. Ushida, K. Toita, Y. Sakai, and T. Tateishi
Hybrid of gel-cultured smooth muscle cells with PLLA sponge as a scaffold towards blood vessel regeneration
Cell Transplant., 11(5), 475-480 (2002). - J. Jiang, N. Kojima, T. Kinoshita, A. Miyajima, W. Yan, and Y. Sakai
Cultivation of fetal mouse liver cells in a three-dimensional poly-L-lactic acid scaffold in the presence of oncostatin M
Cell Transplant.,11(5), 403-406 (2002). - Y. Sakai, J. Jiang, N. Kojima, T. Kinoshita, and A. Miyajima
Enhanced in vitro maturation of fetal liver cells with oncostatin M, nicotinamide and dimethylsulfoxide
Cell Transplant., 11(5), 435-441 (2002). - A. Kamiya, N. Kojima, T. Kinoshita, Y. Sakai, and A. Miyaijma
Maturation of fetal hepatocytes in vitro by extracellular matrices and oncostatin M: induction of tryptophan oxygenase
Hepatology, 35(6), 1351-1359 (2002). - K. Furukawa, T. Ushida, K. Kunii, Y. Sakai, J. Tanaka, and T. Tateishi
Effects of hormone and growth factor on formation of fibroblast-aggregates for tissue-engineered skin
Materials Science & Engineering, C: Biomimetic and Supramolecular Systems, C17(1-2), 59-62 (2001). - K. Naruse, Y. Sakai, L. Guo, Y. Sakamoto, T. Kobayashi, C. Puliatti, G. Aronica, W. Morale, F. Leone, S. Qiang, S.-G. Ming, S. Ming, Z. Li, S.-J. Chang, M. Suzuki, and M. Makuuchi
Efficacy of nonwoven fabric bioreactor immobilized with porcine hepatocytes for ex vivo xenogeneic perfusion treatment of liver failure in dogs
Artificial Organs, 25(4), 273-280 (2001). - Y. Sakai, R. Shoji, B.-S. Kim, A. Sakoda, and M. Suzuki
Cultured human-cell-based bioassays for environmental risk management
Environ. Monit. Assess., 70, 57-70 (2001). - K. Furukawa, T. Ushida, Y. Sakai, K. Kunii, J. Tanaka, and T. Tateishi
Tissue-engineered skin using aggregates of normal human skin fibroblasts and biodegradable material
J. Artif. Organs, 4, 353-356 (2001). - K. S. Furukawa, T. Ushida, Y. Sakai, M. Suzuki, and T. Tateishi
Formation of human fibroblasts-aggregates (spheroids) by rotational culture for tissue-engineered skin
Cell Transplant.,10, 441-445 (2001). - Y. Sakai, K. Furukawa, T. Ushida, T. Tateishi, and M. Suzuki
In vitro organization of biohybrid rat liver tissue incorporating growth-factor- and hormone-releasing biodegradable-polymer microcapsules
Cell Transplant., 10, 479-483 (2001). - Y. Sakai, T. Arai, A. Sakoda, and M. Suzuki
Development of a simple double-layered cell culture system using Caco-2 and TIG-1 cells as a new cytotoxicity test
AATEX, 7(2-3), 47-58 (2001). - R. Shoji, A. Sakoda, Y. Sakai, H. Utsumi, and M. Suzuki
Formulating bioassay data of chemicals and environmental water
Water Sci. and Technol., 42(3-4), 115-124 (2000). - Mantalaris, A., N. Panoskaltsis, Y. Sakai, P. Bourne, C. S. Chang, E. M. Messing, and J.H.D. Wu
Localization of androgen receptor expression in human bone marrow
J. Pathol., 192(5), 361-366, (2000). - R. Shoji, A. Sakoda, Y. Sakai, H. Utsumi, and M. Suzuki
A new assay for hepatotoxicity using LDL-uptaking activity of liver cells
J. Health Sci., 46(6), 493-502 (2000). - R. Shoji, Y. Sakai, A. Sakoda, and M. Suzuki
Development of a rapid and sensitive bioassay device using human cells immobilized in macroporous microcarriers for the on-site evaluation of environmental waters
Appl. Microb. Biotechnol., 54, 432-438 (2000). - Y. Sakai, R. Shoji, Y. Mishima, A. Sakoda, and M. Suzuki
Rapid and sensitive neurotoxicity test based on the morphological changes of PC12 cells with simple computer-assisted image analysis
Journal of Bioscience and Bioengineering, 90(1), 20-24 (2000). - R. Shoji, Y. Sakai, A. Sakoda, and M. Suzuki
Preservation of microplate-attached human hepatoma cells and their use in cytotoxicity tests
Cytotechnology, 32(2), 147-155 (2000).
1991 - 1999
Y. Sakai, K. Naruse, I. Nagashima, T. Muto, and M. Suzuki
A new bioartificial liver using porcine hepatocyte spheroids in high-cell-density suspension perfusion culture: in vitro performance in synthesized culture medium and 100% human plasma
Cell Transplant., 8, 531-541 (1999).- K. Naruse, I. Nagashima, Y. Sakai, G.-X. Jiang, M. Suzuki, T. Muto, and M. Makuuchi
Efficacy of a bioreactor filled with porcine hepatocytes immobilized on non-woven fabrics for ex vivo direct hemoperfusion treatment of liver failure in pigs
Artif. Organs, 22(12), 1031-1037 (1998). - R. Shoji, A. Sakoda, Y. Sakai, M. Suzuki, and H. Utsumi
Rapid bioassay of toxicity in environmental water by LDL-uptaking activity of human cell
Water Science and Technology, 38(7), 271-278 (1998). - A. Sakoda, Y. Sakai, K. Hayakawa, and M. Suzuki
Adsorption of viruses in water environment onto solid surfaces
Water Science and Technology, 35(7), 107-114 (1997). - K. Naruse, Y. Sakai, I. Nagashima, G.-X. Jiang, M. Suzuki, and T. Muto
Comparison of porcine hepatocyte spheroids and single hepatocytes in the non-woven fabric bioartificial liver module
Int. J. Artif. Organs, 19(10), 605-609 (1996). - Y. Sakai, K. Ichikawa, A. Sakoda, and M. Suzuki
Quantitative comparison of rat hepatocyte functions in two improved culture systems with or without rat liver epithelial cell line
Cytotechnology, 21(3), 243-252 (1996). - K. Naruse, Y. Sakai, I. Nagashima, G.-X. Jiang, M. Suzuki, and T. Muto
Development of a new bioartificial liver module filled with porcine hepatocytes immobilized on non-woven fabrics
Int. J. Artif. Organs, 19(6), 347-352 (1996). - K. Ichikawa, Y. Sakai, A. Sakoda, and M. Suzuki
Long-term cytotoxicities of various pesticides evaluated by albumin secretion of primary cultured rat hepatocytes
Biotechnology Techniques, 10(2), 99-102 (1996). - Y. Sakai, K. Naruse, I. Nagashima, T. Muto, and M. Suzuki
Large-scale preparation and functions of porcine hepatocyte spheroids
Int. J. Artif. Organs, 19(5), 294-301 (1996). - Y. Sakai, K. Naruse, I. Nagashima, T. Muto, and M. Suzuki
In vitro function of porcine hepatocyte spheroids in 100% human plasma
Cell Transplant., 5(5S-1), S41-S43, (1996). - Y. Sakai, K. Naruse, I. Nagashima, T. Muto, and M. Suzuki
Short-term hypothermic preservation of porcine hepatocyte spheroids using UW solution
Cell Transplant., 5(4), 501-511 (1996). - Y. Sakai, K. Naruse, I. Nagashima, T. Muto, and M. Suzuki
Functional stability of porcine hepatocyte spheroids in various culture systems under 100% porcine and human plasma conditions
Artificial Organs, 20(1), 56-60 (1996). - Y. Sakai, A. Sakoda, and M. Suzuki
Attachment kinetics of animal cells immediately after contact onto specific and/or non-specific surfaces
Journal of Chemical Engineering of Japan, 28(5), 590-5 (1995). - Y. Sakai and M. Suzuzki
Stable immobilization and functional expressions of hepatocyte spheroids rapidly formed by suspension culture
Artificial Organs Today, 4, 129-140 (1994). - K. Ichikawa, Y. Sakai, A. Sakoda, and M. Suzuki
Primary culture of rat hepatocytes using membrane-supported collagen sandwich with hormone-free medium
Biotechnology Techniques, 8(6), 385-8 (1994). - M. Suzuki, K. Ichikawa, A. Sakoda, and Y. Sakai
Long-term culture of primary rat hepatocytes with high albumin secretion using membrane-supported collagen sandwich
Cytotechnology, 11, 213-218 (1993). - Y. Sakai, K. Furukawa, and M. Suzuki
Immobilization and long-term albumin secretion of hepatocyte spheroids rapidly formed by rotational tissue culture methods
Biotechnology Techniques, 6(6), 527-32 (1992). - Y. Sakai and M. Suzuki
Formation of spheroids of adult rat hepatocytes on polylysine-coated surfaces and their albumin production
Biotechnology Techniques, 5(4), 299-302 (1991).
